| dc.creator | Giambelluca, Miriam Soledad | |
| dc.creator | Ciancio, María Carolina | |
| dc.creator | Orlowski, Alejandro | |
| dc.creator | Gende, Oscar Alfredo | |
| dc.creator | Pouliot, Marc | |
| dc.creator | Aiello, Ernesto Alejandro | |
| dc.date | 2014 | |
| dc.date | 2019-11-08T17:53:52Z | |
| dc.identifier | http://sedici.unlp.edu.ar/handle/10915/85267 | |
| dc.identifier | issn:1015-8987 | |
| dc.description | Background: Bicarbonate transport has crucial roles in regulating intracellular pH (pH<SUB>i</SUB>) in a variety of cells. The purpose of this study was to evaluate its participation in the regulation of pHi in resting and stimulated human neutrophils. Methods: Freshly isolated human neutrophils acidified by an ammonium prepulse were used in this study. Results: We demonstrated that resting neutrophils have a bicarbonate transport mechanism that prevents acidification when the Na<SUP>+</SUP>/H<SUP>+</SUP> exchanger is blocked by EIPA. Neutrophils acidified by an ammonium prepulse showed an EIPA-resistant recovery of pHi that was inhibited by the blocker of the anionic transporters SITS or the Na<SUP>+</SUP>/HCO<SUB>3</SUB>¯ cotransporter (NBC) selective inhibitor S0859, and abolished when sodium was removed from the extracellular medium. In western blot and RT-PCR analysis the expression of NBCe2 but not NBCe1 or NBCn1 was detected in neutrophils Acidified neutrophils increased the EIPA-insensitive pHi recovery rate when its activity was stimulated with fMLF/cytochalasin B. This increase in the removal of acid equivalents was insensitive to the blockade of the NADPH oxidase with DPI. Conclusion: It is concluded that neutrophils have an NBC that regulates basal pH<SUB>i</SUB> and is modulated by chemotactic agents. | |
| dc.description | Facultad de Ciencias Médicas | |
| dc.description | Centro de Investigaciones Cardiovasculares | |
| dc.format | application/pdf | |
| dc.format | 982-990 | |
| dc.language | en | |
| dc.rights | http://creativecommons.org/licenses/by/4.0/ | |
| dc.rights | Creative Commons Attribution 4.0 International (CC BY 4.0) | |
| dc.subject | Ciencias Médicas | |
| dc.subject | Blood cells | |
| dc.subject | Intracellular pH | |
| dc.subject | Ion transport | |
| dc.title | Characterization of the Na+/HCO3¯ cotransport in human neutrophils | |
| dc.type | Articulo | |
| dc.type | Articulo | |
