Artículo de revista
Determination of the potency of hexyl-ciprofloxacin molecules that interact with gold nanoparticles in a reversible manner
Fecha
2019Registro en:
IET Nanobiotechnology, Volumen 13, Issue 3, 2019, Pages 320-325
17518741
10.1049/iet-nbt.2018.5186
Autor
Montero Silva, Francisco
Kogan Bocian, Marcelo
Montenegro, Iván
Guzmán, Fanny
Navarrete, Soledad
Parra, Carolina
Seeger, Michael
Institución
Resumen
This work determined the potency of hexyl-ciprofloxacin molecules that reversibly interact with gold nanoparticles (AuNPs) passivated with 11-mercaptoundecanoic acid (MUA) on Escherichia coli cells. For this, partition of modified antibiotic between different compartments of the gold colloid was determined using analytical techniques. First, concentration of hexyl-ciprofloxacin was determined in the continuous phase of the colloid. Subsequently, the colloid was exposed to a volume of organic immiscible solvent and concentration of the transferred molecules was determined in the organic phase. Comparison of the amount of hexyl-ciprofloxacin in each phase revealed that interaction between molecules and nanoparticles was reversible. Later, this work determined the potency of a population of hexyl-ciprofloxacin molecules contained in a volume of the colloid, and the potency of other population of molecules that only interact with the continuous phase of the colloid. The absolute difference between these two values was proportional to the potency of a number of molecules that interact with the nanoparticles of the colloid.