Artículos de revistas
Nucleolar expression and chromosomal associations in Robertsonian spermatocytes of Mus musculus domesticus
Fecha
2019Registro en:
Genes, Volumen 10, Issue 2, 2019,
20734425
10.3390/genes10020120
Autor
López-Moncada, Fernanda
Tapia, Daniel
Zuñiga, Nolberto
Ayarza, Eliana
López-Fenner, Julio
Redi, Carlo Alberto
Berríos, Soledad
Institución
Resumen
We studied and compared the nucleolar expression or nucleoli from specific bivalents in spermatocytes of the standard Mus musculus domesticus 2n=40, of Robertsonian (Rb) homozygotes 2n = 24 and heterozygotes 2n = 32. We analyzed 200 nuclear microspreads of each specific nucleolar chromosome and spermatocyte karyotype, using FISH to identify specific nucleolar bivalents, immunofluorescence for both fibrillarin of the nucleolus and the synaptonemal complex of the bivalents, and DAPI for heterochromatin. There was nucleolar expression in all the chromosomal conditions studied. By specific nucleolar bivalent, the quantitative relative nucleolar expression was higher in the bivalent 12 than in its derivatives, lower in the bivalent 15 than in its derivatives and higher in the bivalent 16 than its Rb derivatives. In the interactions between non-homologous chromosomal domains, the nucleolar bivalents were preferentially associated throu