Artículos de revistas
The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana
Fecha
2006Registro en:
Biol. Res. v.39 n.2 Santiago 2006
0716-9760
Autor
Urbina, Daniela
Silva, Herman
Meisel, Lee
Institución
Resumen
Thapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering root gravitropism in Arabidopsis thaliana. Thapsigargin concentrations as low as 0.1 µM alter root gravitropism whereas under similar conditions cyclopiazonic acid does not. Furthermore, a fluorescently conjugated thapsigargin (BODIPY FL thapsigargin) suggests that target sites for thapsigargin are located in intracellular organelles in the root distal elongation zone and the root cap, regions known to regulate root gravitropism.