Artículos de revistas
Canine cerebral leishmaniasis: Potential role of matrix metalloproteinase-2 in the development of neurological disease
Fecha
2012-08-15Registro en:
Veterinary Immunology and Immunopathology. Amsterdam: Elsevier B.V., v. 148, n. 3-4, p. 260-266, 2012.
0165-2427
10.1016/j.vetimm.2012.05.007
WOS:000309030400006
1817946671090010
0310405558125634
1817946671090010
Autor
Universidade Estadual Paulista (Unesp)
Institución
Resumen
Matrix metalloproteinases (MMPs) are a group of calcium- and zinc-dependent endopeptidases that are involved in maintaining the extracellular matrix. MMP-2 and MMP-9 are thought to be related to the disruption of the blood-brain-barrier (BBB) by their ability to cleave type IV collagen, the main component of the basal membrane. To establish the presence of MMP-2 and MMP-9 in the pathogenesis of canine cerebral leishmaniasis, we examined the levels of these metalloproteinases in the cerebrospinal fluid (CSF) and serum of dogs with visceral leishmaniasis and neurological symptoms (n = 16) and in the CSF and serum of uninfected healthy dogs (n =10) using zymography. In the CSF of dogs with cerebral leishmaniasis there was a massive presence of active MMP-2, whereas only the levels of both proMMP-2 and proMMP-9 were elevated in the serum. Although the detected MMP activity in the CSF might merely be related to CNS inflammation, these enzymes may also play a collaborative role in the disease progression. Both MMP-2 and MMP-9 are known to target critical constituents of the BBB, and once activated, they may promote cerebral barrier breakdown, allowing the entrance of inflammatory cells and proteins within the nervous system milieu. (c) 2012 Elsevier B.V. All rights reserved.