Artículo de revista
Regulation of protein translation and c-Jun expression by prostate tumor overexpressed 1
Fecha
2014Registro en:
Oncogene, Volumen 33, Issue 9, 2018, Pages 1124-1134
09509232
14765594
10.1038/onc.2013.51
Autor
Marqués, N.
Sesé, M.
Cánovas, V.
Valente, F.
Bermudo, R.
De Torres, I.
Fernández, Y.
Abasolo, I.
Fernández, P. L.
Contreras, H.
Castellón, E.
Celià-Terrassa, T.
Méndez, R.
Ramón Y Cajal, S.
Thomson, T. M.
Paciucci, R.
Institución
Resumen
Prostate tumor overexpressed-1 (PTOV1), a modulator of the Mediator transcriptional regulatory complex, is expressed at high levels in prostate cancer and other neoplasias in association with a more aggressive disease. Here we show that PTOV1 interacts directly with receptor of activated protein C kinase 1 (RACK1), a regulator of protein kinase C and Jun signaling and also a component of the 40S ribosome. Consistent with this interaction, PTOV1 was associated with ribosomes and its overexpression promoted global protein synthesis in prostate cancer cells and COS-7 fibroblasts in a mTORC1-dependent manner. Transfection of ectopic PTOV1 enhanced the expression of c-Jun protein without affecting the levels of c-Jun or RACK1 mRNA. Conversely, knockdown of PTOV1 caused significant declines in global protein synthesis and c-Jun protein levels. High levels of PTOV1 stimulated the motility and invasiveness of prostate cancer cells, which required c-Jun, whereas knockdown of PTOV1 strongly inhi