Artículo de revista
Human papillomavirus genotyping of cervical uterine preneoplastic lesions in a high risk area Detección y tipificación de virus papiloma humano en lesiones preneoplásicas de cuello uterino
Fecha
2010Registro en:
Revista Medica de Chile, Volumen 138, Issue 11, 2018, Pages 1343-1350
07176163
00349887
Autor
López M., Jaime
Ili, Carmen G.
Brebi, Priscilla
García M., Patricia
Capurro V., Italo
Guzmán G., Pablo
Suárez P., Eugenio
Ojeda F., José Manuel
Roa S., Juan Carlos
Institución
Resumen
Background: The relationship between human papillomavirus (HPV) and uterine cervical cancer (UCC) is widely known and accepted. Aim: To determine the frequency of genotypes of HPV in cervical preneoplastic lesions in a high risk area of UCC. Material and Methods: Using a combination of PCR and Reverse Line Blot technique, 235 formalin fixed paraffin embedded samples, with diagnosis of low-grade squamous intraepithelial lesion (LSIL) or high-grade squamous intraepithelial lesion (HSIL) were genotyped. Results: HPV was detected in 61.2% of LSIL and 78.1% of HSIL. The main genotypes found were HPV 16, 18, 31, 45, 56 y 58. HPV 16 was the most common in both LSIL (18.1%) and HSIL (36.9%). HPV 16 or 18 were present in 25.1% and 47.1% of the LSIL and HSIL respectively. In both LSIL and HSIL, the predominant viral genotypes were those types classified as with a high oncogenic risk. Conclusions: HPV genotypes 16, 18, 31, 45, 56 y 58 were the most common in our series. HPV 16 and 18, viral types