Artículo de revista
Identification of peptides in flowers of Sambucus nigra with antimicrobial activity against aquaculture pathogens
Fecha
2018-05Registro en:
Molecules, 23(5), Mayo 2018. 1033.
1420-3049
10.3390/molecules23051033
Autor
Álvarez, Claudio
Barriga, Andrés
Albericio, Fernando
Romero, María
Guzman, Fanny
Institución
Resumen
The elder (Sambucus spp.) tree has a number of uses in traditional medicine. Previous studies have demonstrated the antimicrobial properties of elderberry liquid extract against human pathogenic bacteria and also influenza viruses. These properties have been mainly attributed to phenolic compounds. However, other plant defense molecules, such as antimicrobial peptides (AMPs), may be present. Here, we studied peptide extracts from flowers of Sambucus nigra L. The mass spectrometry analyses determined peptides of 3 to 3.6 kDa, among them, cysteine-rich peptides were identified with antimicrobial activity against various Gram-negative bacteria, including recurrent pathogens of Chilean aquaculture. In addition, membrane blebbing on the bacterial surface after exposure to the cyclotide was visualized by SEM microscopy and SYTOX Green permeabilization assay showed the ability to disrupt the bacterial membrane. We postulate that these peptides exert their action by destroying the bacterial membrane.