Artículos de revistas
Deltamethrin Binding to Triatoma infestans (Hemiptera: Reduviidae) Lipoproteins. Analysis by Solvent Bar Microextraction Coupled to Gas Chromatography
Fecha
2015-11Registro en:
Dulbecco, Andrea Belen; Mijailovsky, Sergio Javier; Girotti, Juan Roberto; Juarez, Marta Patricia; Deltamethrin Binding to Triatoma infestans (Hemiptera: Reduviidae) Lipoproteins. Analysis by Solvent Bar Microextraction Coupled to Gas Chromatography; Entomological Society of America; Journal of Medical Entomology; 52; 6; 11-2015; 1254-1259
0022-2585
1938-2928
CONICET Digital
CONICET
Autor
Dulbecco, Andrea Belen
Mijailovsky, Sergio Javier
Girotti, Juan Roberto
Juarez, Marta Patricia
Resumen
The binding of deltamethrin (DLM) to the hemipteran Triatoma infestans (Klug) hemolymph lipoproteins was evaluated in vitro. After DLM incubation with the insect hemolymph, lipoproteins were fractioned by ultracentrifugation. DLM binding was analyzed by a microextractive technique—solvent bar microextraction—a solventless methodology to extract DLM from each lipoprotein fraction. This is a novel use of the technique applied to extract an insecticide from an insect fluid. Capillary gas chromatography with microelectron capture detection was used to detect DLM bound by the T. infestans hemolymph lipoproteins and to identify the preferred DLM carrier. We show that Lp and VHDLp I lipoproteins are mainly responsible for DLM transport in T. infestans, both in DLM-resistant and DLM-susceptible bugs. Our results also indicate that DLM amounts transported are not related to DLM susceptibility.