Artículos de revistas
Cloning and sequencing of c-DNA encoding N-methyl-D-aspartate receptor
Ruiz E., Ana Z.
The objective of the present study was to report the molecular cloning and determination of the sequences N-methyl-D-aspartate subunit-1 receptor (NMDAR-1) in the hypothalamus of sheep. The re-amplified DNA template for NMDA -1 from the hypothalamus of male sheep was cloned using pBluescript-Sk-plasmids (pBSK, 2958 bp). Purified plasmids containing the NMDA receptor c-DNA were sequenced using the dye-terminator chemistry at the University of Nebraska- Lincoln, Nebraska, USA. Results were entered into the National Center for Biotechnology Web site as accession number AY434689. The sequence of Ovis aries (sheep) NMDAR-1 mRNA from hypothalamus of male sheep has 94-97% homology with Homo sapiens, 94-100% homology with Sus scrofa, and 88-90% homology with Rattus norvegicus NMDAR-1 mRNA sequence, and 94-97% homology with Homo sapiens glutamate receptor and transcript variant NR1-2 and NR1-1 mRNA sequence. These results show high evolutionary conservation of NMDA receptor subunit-1 across species.Special thanks to Consejo de Desarrollo Científico y Humanístico (CDCH), at Universidad Central de Venezuela (UCV), Venezuela, for financing Ph.D studies in the University of Nebraska, Lincoln, USA. The author wishes to express her gratitude to her tutor, Dr. Roger Kittok, who was abundantly helpful and offered invaluable assistance, support and guidance, and to Cederberg Rebecca for assisting me in molecular cloning.