dc.creator | Noya, Verónica | |
dc.creator | Brossard, Natalie | |
dc.creator | Rodríguez, Ernesto | |
dc.creator | Dergan Dylon, Leonardo Sebastian | |
dc.creator | Carmona, Carlos | |
dc.creator | Rabinovich, Gabriel Adrián | |
dc.creator | Freire, Teresa | |
dc.date.accessioned | 2018-11-05T23:39:08Z | |
dc.date.accessioned | 2018-11-06T12:06:29Z | |
dc.date.available | 2018-11-05T23:39:08Z | |
dc.date.available | 2018-11-06T12:06:29Z | |
dc.date.created | 2018-11-05T23:39:08Z | |
dc.date.issued | 2017-01-12 | |
dc.identifier | Noya, Verónica; Brossard, Natalie; Rodríguez, Ernesto; Dergan Dylon, Leonardo Sebastian; Carmona, Carlos; et al.; A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity; Nature Publishing Group; Scientific Reports; 7; 12-1-2017; 1-12 | |
dc.identifier | 2045-2322 | |
dc.identifier | http://hdl.handle.net/11336/63727 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1863158 | |
dc.description.abstract | Fasciolosis is a trematode zoonosis of interest in public health and cattle production. We report here the immunostimulatory effect of a 66 mer mucin-like peptide from Fasciola hepatica (Fhmuc), which synergizes with lipopolysaccharide (LPS) to promote dendritic cell (DC) maturation, endowing these cells with Th1-polarizing capacity. Exposure of DCs to Fhmuc in presence of LPS induced enhanced secretion of pro-inflammatory cytokines and expression of co-stimulatory molecules by DCs, promoting their T cell stimulatory capacity and selectively augmenting IFN- secretion by allogeneic T cells. Furthermore, exposure of DCs to Fhmuc augmented LPS-induced Toll-like receptor (TLR) 4 expression on the cell surface. Finally, Fhmuc-conditioned DCs induced parasite specific-adaptive immunity with increased levels of IFN-gamma secreted by splenocytes from vaccinated animals, and higher parasite-specific IgG antibodies. However, DC-treated Fhmuc conferred modest protection against F. hepatica infection highlighting the potent immuno-regulatory capacity of the parasite. In summary, this work highlights the capacity of a mucin-derived peptide from F. hepatica to enhance LPS-maturation of DCs and induce parasite-specific immune responses with potential implications in vaccination and therapeutic strategies. | |
dc.language | eng | |
dc.publisher | Nature Publishing Group | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/srep40615 | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/srep40615 | |
dc.relation | info:eu-repo/semantics/altIdentifier/pmid/28079156 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | HELMINTHS | |
dc.subject | MUCIN | |
dc.subject | DENDRITIC CELLS | |
dc.subject | IMMUNOGENICITY | |
dc.title | A mucin-like peptide from Fasciola hepatica instructs dendritic cells with parasite specific Th1-polarizing activity | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |
dc.type | Artículos de revistas | |