dc.creator | Crespo, Maria Ines | |
dc.creator | Zacca, Estefanía | |
dc.creator | Núñez, Nicolás | |
dc.creator | Ranocchia, Romina Paola | |
dc.creator | Maccioni, Mariana | |
dc.creator | Maletto, Belkys Angélica | |
dc.creator | Pistoresi, Maria Cristina | |
dc.creator | Moron, Victor Gabriel | |
dc.date.accessioned | 2017-10-26T20:13:02Z | |
dc.date.available | 2017-10-26T20:13:02Z | |
dc.date.created | 2017-10-26T20:13:02Z | |
dc.date.issued | 2013-01 | |
dc.identifier | Crespo, Maria Ines; Zacca, Estefanía; Núñez, Nicolás; Ranocchia, Romina Paola; Maccioni, Mariana; et al.; TLR7 triggering with Polyuridylic acid promotes cross-presentation in CD8a+ conventional dendritic cells by enhancing antigen preservation and MHC Class I antigen permanence on the dendritic cell surface; American Association of Immunologists; Journal of Immunology; 190; 3; 1-2013; 948-960 | |
dc.identifier | 0022-1767 | |
dc.identifier | http://hdl.handle.net/11336/27145 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.description.abstract | ssRNA can interact with dendritic cells (DCs) through binding to TLR7, inducing secretion of proinflammatory cytokines and type I IFN. Triggering TLR7 enhances cross-priming of CD8+ T cells, which requires cross-presentation of exogenous Ag to DCs. However, how TLR triggering can affect Ag cross-presentation is still not clear. Using OVA as an Ag model, we observed that stimulation of TLR7 in DCs by polyuridylic acid (polyU), a synthetic ssRNA analog, generates a strong specific cytotoxic response in C57BL/6 mice. PolyU stimulate CD8a+ DCs to cross-prime naive CD8+ T cells in a type I IFN–dependent fashion. This enhanced cross-priming is accompanied by a higher density of OVA256-264/H-2Kb complexes on CD8a+ DCs treated with polyU, as well as by upregulation of costimulatory molecules and increased secretion of proinflammatory cytokines by DCs. Crosspriming of CD8+ T cells by DCs treated with polyU requires proteasome and Ag translocation to cytosol through the Sec61 channel in DCs. The observed enhancement in OVA cross-presentation with polyU in DCs could be mediated by a limited Ag degradation in endophagosomal compartments and a higher permanence of OVA peptide/MHC class I complexes on DCs. These observations clearly reveal that key steps of Ag processing for cross-presentation can be modulated by TLR ligands, opening new avenues for understanding their mechanisms as adjuvants of the immune response. | |
dc.language | eng | |
dc.publisher | American Association of Immunologists | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4049/jimmunol.1102725 | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://www.jimmunol.org/content/190/3/948 | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | Dendritic Cell | |
dc.subject | Tlr7 | |
dc.subject | Polyu | |
dc.subject | Cross-Presentation | |
dc.title | TLR7 triggering with Polyuridylic acid promotes cross-presentation in CD8a+ conventional dendritic cells by enhancing antigen preservation and MHC Class I antigen permanence on the dendritic cell surface | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |