dc.creator | Vilela, Carolina Assis Pereira | |
dc.creator | Souza, Lucas Eduardo Botelho de | |
dc.creator | Siqueira, Rubens Camargo | |
dc.creator | Rodrigues, Rodrigo do Tocantins Calado de Saloma | |
dc.creator | Covas, Dimas Tadeu | |
dc.creator | Paula, Jayter Silva de | |
dc.date.accessioned | 2018-06-17T04:37:35Z | |
dc.date.accessioned | 2018-07-04T17:14:56Z | |
dc.date.available | 2018-06-17T04:37:35Z | |
dc.date.available | 2018-07-04T17:14:56Z | |
dc.date.created | 2018-06-17T04:37:35Z | |
dc.date.issued | 2018 | |
dc.identifier | Stem Cell Research & Therapy. 2018 Jun 13;9(1):155 | |
dc.identifier | http://www.producao.usp.br/handle/BDPI/51629 | |
dc.identifier | 10.1186/s13287-018-0909-y | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1646666 | |
dc.description.abstract | Abstract
Background
Bone marrow-derived mesenchymal stromal cell (MSC) therapy is a promising treatment for several degenerative ocular diseases; however, no reproducible method of monitoring these cells into the eye has been established. The aim of this study was to describe successful bioluminescence imaging (BLI) to detect viable luciferase-expressing MSC in the eye.
Methods
Human donor MSC in culture were transduced with 50 μl luciferase lentiviral vector (three viral particles/cell) prior to intraocular injection. Twenty-one right eyes of 21 rabbits were evaluated through BLI after receiving 1 × 106 luciferase-expressing MSC intravitreally. Contralateral eyes were injected with vehicle (phosphate-buffered saline (PBS)) and were used as controls. At seven different time points (1 h to 60 days), d-luciferin (40 mg/ml, 300 μl PBS) was injected in subsets of six enucleated eyes for evaluation of radiance decay through BLI analysis. CD90 and CD73 immunofluorescence was studied in selected eyes.
Results
Eyes injected with MSC showed high BLI radiance immediately after d-luciferin injection and progressive decay until 60 days. Mean BLI radiance measures from eyes with luciferase-expressing MSC were significantly higher than controls from 8 h to 30 days. At the thirtieth day, positive CD90- and CD73-expressing cells were observed only in the vitreous cavity of eyes injected with MSC.
Conclusions
Viable MSC were identified in the vitreous cavity 1 month after a single injection. Our results confirmed BLI as a useful and reliable method to detect MSC injected into the eye globe. | |
dc.language | eng | |
dc.publisher | BioMed Central | |
dc.relation | Stem Cell Research and Therapy | |
dc.rights | The Author(s). | |
dc.rights | openAccess | |
dc.subject | Bioluminescence | |
dc.subject | Cell transplantation | |
dc.subject | Cell viability assessment | |
dc.subject | Intravitreal injection | |
dc.subject | Luciferase | |
dc.subject | Mesenchymal stromal cell | |
dc.title | Ex vivo evaluation of intravitreal mesenchymal stromal cell viability using bioluminescence imaging | |
dc.type | Artículos de revistas | |