Artículos de revistas
Isolation of a lectin and a galactoxyloglucan from Mucuna sloanei seeds
Fecha
2009Registro en:
PHYTOCHEMISTRY, v.70, n.17-18, p.1965-1972, 2009
0031-9422
10.1016/j.phytochem.2009.08.011
Autor
TEIXEIRA-SA, Daniele M. A.
REICHER, Fany
BRAGA, Renata C.
BELTRAMINI, Leila Maria
MOREIRA, Renato de Azevedo
Institución
Resumen
A lectin and a galactoxyloglucan were characterized from Mucuna sloanei seed cotyledons. The galactoxyloglucan, isolated by water extraction and ethanol precipitation, had Glc:Xyl:Gal proportions in a molar ratio of 1.8:1.7:1.0 and a molar mass (M(w)) of 1.6 x 10(6) g mol(-1). The lectin (sloanin), isolated from the same seed by affinity chromatography on cross-linked Adenanthera pavonina galactomannan, gave two protein bands by SDS-PAGE (36 and 34 kDa) and one peak by gel filtration (63.6 kDa). Its N-terminal sequence indicated similar to 69% identity with soybean agglutinin to leguminous lectins. Circular dichroism (CD) spectra established that sloanin predominantly contains beta-sheet structures. Sloanin has similar to 5.5% carbohydrate and displayed hemagglutinating activity against rabbit and enzyme treated human erythrocytes, inhibited only by D-Gal containing sugars. The interaction between sloanin and storage cell-wall galactoxyloglucan was tested by affinity chromatography and fluorescence spectroscopy. (C) 2009 Elsevier Ltd. All rights reserved.