Artículos de revistas
HPLC-UV determination of metformin in human plasma for application in pharmacokinetics and bioequivalence studies
Fecha
2008Registro en:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.46, n.1, p.143-147, 2008
0731-7085
10.1016/j.jpba.2007.10.007
Autor
PORTA, Valentina
SCHRAMM, Simone Grigoleto
KANO, Eunice Kazue
KOONO, Eunice Emiko
ARMANDO, Yara Popst
FUKUDA, Kazuo
SERRA, Cristina Helena dos Reis
Institución
Resumen
In this study, a simple, rapid and sensitive HPLC method with UV detection is described for determination of metformin in plasma samples from bioequivalence assays. Sample preparation was accomplished through protein precipitation with acetonitrile and chromatographic separation was performed on a reversed-phase phenyl column at 40 degrees C. Mobile phase consisted of a mixture of phosphate buffer and acetonitrile at flow rate of 1.0 ml/min. Wavelength was set at 236 nm. The method was applied to a bioequivalence study of two drug products containing metformin, and allowed determination of metformin at low concentrations with a higher throughput than previously described methods. (c) 2007 Elsevier B.V. All rights reserved.