Artículos de revistas
Clavulanic Acid Separation On Fixed Bed Columns Of Layered Double Hydroxides: Optimization Of Operating Parameters Using Breakthrough Curves
Registro en:
Process Biochemistry. Elsevier Sci Ltd, v. 51, p. 509 - 516, 2016.
1359-5113
1873-3298
WOS:000373419700006
10.1016/j.procbio.2016.01.011
Autor
Soares Forte
Marcus Bruno; Taviot-Gueho
Christine; Leroux
Fabrice; Rodrigues
Maria Isabel; Maugeri Filho
Francisco
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The adsorption of clavulanic acid (CA) in a fixed-bed column of layered double hydroxides (LDHME) was investigated. Breakthrough curves were obtained experimentally and the system was evaluated with regards to column operation time, efficiency and productivity as functions of simultaneous variations of superficial velocity (v(z)) and bed height (L) using a central composite rotatable design (CCRD). At the optimized condition (v(z) = 1.00 cm/min and L = 6.5 cm), the responses were: 46 min, 146 min, 100 min and 13 kg/h cm(3), for breakthrough time (t(b)), exhaustion time (t(e)), the difference t(e) - t(b) and productivity (P), respectively. These results represent no change in t(b), but a 50% decrease of t(e), 40% decrease of (t(e) - t(b)) and 38% increase of P, which is advantageous for the process. The factorial design technique was shown to be an efficient tool for assessing factor influences and was effective in optimizing the column operating conditions. Good separation of CA from the amino acids tyrosine (TYR) and proline (PRO) was observed in fixed bed columns. The LDHME adsorbent was shown to be an excellent alternative for CA purification. (C) 2016 Elsevier Ltd. All rights reserved. 51 4 509 516 FAPESP (Foundation for Research Support of the State of sao Paulo) [2008/57298-3] CAPES (Coordination of Improvement of Higher Education Personnel) [BEX 6843/10-7] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)