Artículos de revistas
Polyphenol-rich Propolis Extracts From China And Brazil Exert Anti-inflammatory Effects By Modulating Ubiquitination Of Traf6 During The Activation Of Nf-kappa B
Registro en:
Polyphenol-rich Propolis Extracts From China And Brazil Exert Anti-inflammatory Effects By Modulating Ubiquitination Of Traf6 During The Activation Of Nf-kappa B. Elsevier Science Bv, v. 19, p. 464-478 DEC-2015.
1756-4646
WOS:000365634100043
10.1016/j.jff.2015.09.009
Autor
Wang
Kai; Hu
Lin; Jin
Xiao-Lu; Ma
Quan-Xin; Marcucci
Maria Cristina; Lagareiro Netto
Amandio Augusto; Helena Frankland Sawaya
Alexandra Christine; Huang
Shuai; Ren
Wen-Kai; Conlon
Michael A.; Topping
David L.; Hu
Fu-Liang
Institución
Resumen
Propolis has documented anti-inflammatory properties, although its mechanisms of action are poorly understood. In this study, the anti-inflammatory effects of polyphenol-rich propolis extracts (PPE) from China (CPPE) and Brazil (BPPE) were examined. Oral administration of PPE to lipopolysaccharide (LPS)-challenged mice decreased serum proinflammatory cytokine concentrations and inhibited pulmonary nuclear factor (NF)-kappa B activation. Both PPE types modulated LPS-induced key inflammatory mediators production in RAW 264.7 macrophages. They also suppressed NF-kappa B activation in HEK 293T cells, correlating well with their inhibitory effects on I kappa B phosphorylation and p65 nuclear translocation in LPS-activated macrophages. We found PPE suppressed NF-kappa B activation through delaying the ubiquitination of TRAF6 in HeLa-T6RZC stable cells and by directly disrupting the polyubiquitin synthesis in an in vitro kinase assay system. Overall, analysis showed substantial compositional differences between CPPE and BPPE; nevertheless, they both displayed similar anti-inflammatory properties through NF-kappa B-responsive inflammatory gene expressions by inhibiting TRAF6 dependent canonical NF-kappa B pathway. (C) 2015 Elsevier Ltd. All rights reserved. 19
464 478 National Natural Science Foundation of China [31272512]