Establishing The Feasibility Of Using β-glucosidase Entrapped In Lentikats And In Sol-gel Supports For Cellobiose Hydrolysis.

dc.creatorFigueira, Joelise A
dc.creatorSato, Hélia H
dc.creatorFernandes, Pedro
dc.date2013-Jan
dc.date2015-11-27T13:31:12Z
dc.date2015-11-27T13:31:12Z
dc.date.accessioned2018-03-29T01:17:00Z
dc.date.available2018-03-29T01:17:00Z
dc.identifierJournal Of Agricultural And Food Chemistry. v. 61, n. 3, p. 626-34, 2013-Jan.
dc.identifier1520-5118
dc.identifier10.1021/jf304594s
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23294439
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200431
dc.identifier23294439
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300664
dc.descriptionβ-Glucosidases represent an important group of enzymes due to their pivotal role in various biotechnological processes. One of the most prominent is biomass degradation for the production of fuel ethanol from cellulosic agricultural residues and wastes, where the use of immobilized biocatalysts may prove advantageous. Within such scope, the present work aimed to evaluate the feasibility of entrapping β-glucosidase in either sol-gel or in Lentikats supports for application in cellobiose hydrolysis, and to perform the characterization of the resulting bioconversion systems. The activity and stability of the immobilized biocatalyst over given ranges of temperature and pH values were assessed, as well as kinetic data, and compared to the free form, and the operational stability was evaluated. Immobilization increased the thermal stability of the enzyme, with a 10 °C shift to an optimal temperature in the case of sol-gel support. Mass transfer hindrances as a result of immobilization were not significant, for sol-gel support. Lentikats-entrapped glucosidase was used in 19 consecutive batch runs for cellobiose hydrolysis, without noticeable decrease in product yield. Moreover, encouraging results were obtained for continuous operation. In the overall, the feasibility of using immobilized biocatalysts for cellobiose hydrolysis was established.
dc.description61
dc.description626-34
dc.languageeng
dc.relationJournal Of Agricultural And Food Chemistry
dc.relationJ. Agric. Food Chem.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBiomass
dc.subjectCellobiose
dc.subjectEnzymes, Immobilized
dc.subjectFeasibility Studies
dc.subjectGels
dc.subjectHydrogen-ion Concentration
dc.subjectHydrolysis
dc.subjectMicroscopy, Electron, Scanning
dc.subjectPolymethyl Methacrylate
dc.subjectTemperature
dc.subjectBeta-glucosidase
dc.titleEstablishing The Feasibility Of Using β-glucosidase Entrapped In Lentikats And In Sol-gel Supports For Cellobiose Hydrolysis.
dc.typeArtículos de revistas


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