Evaluation Of Genotypic Diversity Of Streptococcus Mutans Using Distinct Arbitrary Primers.

dc.creatorTabchoury, Cínthia Pereira Machado
dc.creatorSousa, Maria Clara K
dc.creatorArthur, Rodrigo Alex
dc.creatorMattos-Graner, Renata Oliveira
dc.creatorDel Bel Cury, Altair Antoninha
dc.creatorCury, Jaime Aparecido
dc.date
dc.date2015-11-27T13:13:24Z
dc.date2015-11-27T13:13:24Z
dc.date.accessioned2018-03-29T01:07:52Z
dc.date.available2018-03-29T01:07:52Z
dc.identifierJournal Of Applied Oral Science : Revista Fob. v. 16, n. 6, p. 403-7
dc.identifier1678-7765
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19082399
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198072
dc.identifier19082399
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298305
dc.descriptionStreptococcus mutans has been considered one of the main etiological agents of dental caries and the genotypic diversity rather than its salivary counts may be considered as a virulence factor of this bacterium. For genotyping with polymerase chain reaction (PCR) with arbitrary primers, several primers have been used in order to improve complexity and specificity of amplicon patterns. Thus, the aim of this study was to evaluate the degree of agreement of genotypic identification among AP-PCR reactions performed with 5 distinct arbitrary primers of S. mutans isolated from saliva. Stimulated saliva was collected from 11 adult volunteers for isolation of S. mutans, and a total of 88 isolates were genotyped with arbitrary primers OPA 02, 03, 05, 13 and 18. Fourteen distinct genotypes were identified in the saliva samples. Most volunteers (9 out of 11) presented only one genotype. The results of the present study suggest that primers OPA 02, 03, 05 and 13 were suitable for genotypic identification of S. mutans isolates of saliva from adult volunteers.
dc.description16
dc.description403-7
dc.languageeng
dc.relationJournal Of Applied Oral Science : Revista Fob
dc.relationJ Appl Oral Sci
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAdult
dc.subjectBacteriological Techniques
dc.subjectDna Primers
dc.subjectDna, Bacterial
dc.subjectElectrophoresis, Agar Gel
dc.subjectEthidium
dc.subjectFluorescent Dyes
dc.subjectGenetic Variation
dc.subjectGenotype
dc.subjectHumans
dc.subjectPolymerase Chain Reaction
dc.subjectRandom Amplified Polymorphic Dna Technique
dc.subjectSaliva
dc.subjectStreptococcus Mutans
dc.subjectVirulence
dc.titleEvaluation Of Genotypic Diversity Of Streptococcus Mutans Using Distinct Arbitrary Primers.
dc.typeArtículos de revistas


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