Comparison of lipase production on crambe oil and meal by Fusarium sp (Gibberella fujikuroi complex)

dc.creatorOliveira, BH
dc.creatorCoradi, GV
dc.creatorAttili-Angelis, D
dc.creatorScauri, C
dc.creatorLuques, AHPG
dc.creatorBarbosa, AM
dc.creatorDekker, RFH
dc.creatorNeto, PO
dc.creatorLima, VMG
dc.date2013
dc.dateDEC
dc.date2014-07-30T14:00:12Z
dc.date2015-11-26T17:44:37Z
dc.date2014-07-30T14:00:12Z
dc.date2015-11-26T17:44:37Z
dc.date.accessioned2018-03-29T00:26:49Z
dc.date.available2018-03-29T00:26:49Z
dc.identifierEuropean Journal Of Lipid Science And Technology. Wiley-blackwell, v. 115, n. 12, n. 1413, n. 1425, 2013.
dc.identifier1438-7697
dc.identifier1438-9312
dc.identifierWOS:000328733600008
dc.identifier10.1002/ejlt.201300087
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56259
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56259
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287931
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionThis study provides a comparison of fermentation conditions for the production lipases by Fusarium sp. (Gibberella fujikuroi complex) isolate FCLA-MA-41 in submerged (SmF) and solid-state fermentation (SSF) using agro-industrial residues. To assess SmF, a univariate study of seven carbon sources (crambe, corn, linseed, olive, palm (dende), soybean oils and chicken fat) and four nitrogen sources (ammonium sulfate, sodium nitrate, urea, yeast extract) was performed. The relationship among the concentrations of carbon and nitrogen sources, Triton X-100 and yeast extract was studied using a Central Composite Design (CCD). To assess SSF, different residues (sugarcane bagasse) and oil-seed meals (castorbean, corn, crambe, soybean) with additional supplements added (minimum salts, seed oils) were examined. Based on the CCD matrix, a medium containing crambe oil (17.5mL/L), Triton X-100 (5g/L), ammonium sulfate (5g/L) and yeast extract (1g/L) was proposed, resulting in a lipase titer of 3.0 +/- 0.25U/mL. The cost/production relationship was 7.73% less expensive than SmF with corn oil. SSF produced a maximum lipase titer of 5.0 +/- 0.25U/gds on crambe meal moistened with phosphate buffer. The best cost/enzyme activity estimate was for SSF with crambe meal as substrate in only distilled water (87.27% less expensive than SmF).
dc.description115
dc.description12
dc.descriptionSI
dc.description1413
dc.description1425
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFAPESP [2010/07998-9]
dc.languageen
dc.publisherWiley-blackwell
dc.publisherHoboken
dc.publisherEUA
dc.relationEuropean Journal Of Lipid Science And Technology
dc.relationEur. J. Lipid Sci. Technol.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectCentral composite design
dc.subjectCorn oil
dc.subjectChicken fat
dc.subjectAmmonium sulfate
dc.subjectTriton X-100
dc.subjectSolid-state Fermentation
dc.subjectYarrowia-lipolytica
dc.subjectPenicillium-aurantiogriseum
dc.subjectExtracellular Lipase
dc.subjectBacterial Lipases
dc.subjectOlive Oil
dc.subjectPurification
dc.subjectAbyssinica
dc.subjectOxysporum
dc.subjectWaste
dc.titleComparison of lipase production on crambe oil and meal by Fusarium sp (Gibberella fujikuroi complex)
dc.typeArtículos de revistas


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