INHIBITION OF THE WHEAT-GERM DNA POLYMERASE-A ACTIVITY BY THE ANTIVIRAL DRUG HPA-23

dc.creatorAOYAMA, H
dc.creatorJUCA, MB
dc.creatorCASTROVIEJO, M
dc.creatorLITVAK, S
dc.date1993
dc.dateDEC
dc.date2014-12-16T11:35:30Z
dc.date2015-11-26T17:30:17Z
dc.date2014-12-16T11:35:30Z
dc.date2015-11-26T17:30:17Z
dc.date.accessioned2018-03-29T00:17:15Z
dc.date.available2018-03-29T00:17:15Z
dc.identifierPlant Molecular Biology. Kluwer Academic Publ, v. 23, n. 5, n. 1055, n. 1060, 1993.
dc.identifier0167-4412
dc.identifierWOS:A1993MM84200011
dc.identifier10.1007/BF00021819
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/80641
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/80641
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/80641
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285485
dc.descriptionWheat germ DNA polymerase A, a gamma-like enzyme, recognized efficiently natural and synthetic RNA templates, resembling a retroviral reverse transcriptase (P. Laquel et al., Biochim Biophys Acta 1048 (1990):139-148). Ammonium-21-tungsto-9-antimoniate (HPA-23), an antiviral drug, inhibited the DNA polymerase A activities, independently of the template primers used, i.e. activated DNA or polyriboadenylic acid oligodeoxythymidylate (poly(rA)-oligo(dT)). The inhibition observed in the poly(rA)-oligo(dT)-directed DNA polymerase A activity occurred in the presence of either Mg2+, or Mn2+ as divalent cation, and also with the 2'-fluoro analogue of poly(rA) as template. HPA-23 was a noncompetitive inhibitor with respect to TTP, activated DNA, poly(rA)-oligo(dT), and poly(dAfl)-oligo(dT). A preincubation study showed a reversible HPA-23 binding to DNA polymerase A, in the presence of poly(rA)-oligo(dT) as the template primer.
dc.description23
dc.description5
dc.description1055
dc.description1060
dc.languageen
dc.publisherKluwer Academic Publ
dc.publisherDordrecht
dc.publisherHolanda
dc.relationPlant Molecular Biology
dc.relationPlant Mol.Biol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectDNA POLYMERASE-A
dc.subjectHPA-23
dc.subjectINHIBITION
dc.subjectWHEAT GERM
dc.subjectReverse-transcriptase
dc.subjectAids
dc.subjectTemplates
dc.subjectEmbryos
dc.subjectGamma
dc.subjectAcid
dc.titleINHIBITION OF THE WHEAT-GERM DNA POLYMERASE-A ACTIVITY BY THE ANTIVIRAL DRUG HPA-23
dc.typeArtículos de revistas


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