Structural and biological characterization of a crotapotin isoform isolated from Crotalus durissus cascavella venom

dc.creatorde Oliveira, DG
dc.creatorToyama, MH
dc.creatorMartins, AMC
dc.creatorHavt, A
dc.creatorNobre, ACL
dc.creatorMarangoni, S
dc.creatorCamara, PR
dc.creatorAntunes, E
dc.creatorde Nucci, G
dc.creatorBeliam, LOS
dc.creatorFonteles, MC
dc.creatorMonteiro, HSA
dc.date2003
dc.dateJUL
dc.date2014-11-19T23:55:55Z
dc.date2015-11-26T17:09:49Z
dc.date2014-11-19T23:55:55Z
dc.date2015-11-26T17:09:49Z
dc.date.accessioned2018-03-28T23:58:26Z
dc.date.available2018-03-28T23:58:26Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 42, n. 1, n. 53, n. 62, 2003.
dc.identifier0041-0101
dc.identifierWOS:000184753800008
dc.identifier10.1016/S0041-0101(03)00100-4
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79631
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79631
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79631
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1280715
dc.descriptionEnvenoming by Crotalus durissus subspecies leads to coagulation disorders, myotoxicity, neurotoxicity and acute renal failure. The most serious systemic alteration and primary cause of death after snakebite is acute renal failure. In this work, we isolated crotapotin, an acid component (Crtp) of crotoxin from Crotalus durissus cascavella venom and we investigated its bactericidal and pro-inflammatory activities as well as its renal effects in rat isolated perfused kidneys. Crtp was bactericidal to the Gram-negative species Xanthomonas axonopodis pv. passiflorae, but was less effective against the Gram-positive Claribacteri ssp, probably because of differences in the cell wall composition. Crtp showed a high amino acid sequence homology with other Crtps described in the literature (around of 90%) and its A and B chains had high conserved regions corresponding to the calcium-binding loop, catalytic site and helix 3 of PLA(2). The Crtp showed moderate pro-inflammatory activity and increased significantly the inflammation evoked by PLA(2) when co-injected or co-incubated with PLA(2). The renal parameters evaluated included the perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF), glomerular filtration rate (GFR) and percent of sodium tubular transport (%TNa+). Crotapotin (5 mug/ml) significantly increased the PP and RVR, whereas the GFR, UF and %TNa+ were unaffected. These results suggest that crotoxin is the main venom component responsible for nephrotoxicity and crotapotin contributes little to this phenomenom. The biological and bactericidal actions of Crtp also suggest that this protein may have functions other than simply acting as a chaperone for PLA(2). (C) 2003 Elsevier Science Ltd. All rights reserved.
dc.description42
dc.description1
dc.description53
dc.description62
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectCrotalus durissus cascavella
dc.subjectCrotapotin isoform
dc.subjectPLA(2)
dc.subjectvenom
dc.subjectrattlesnake
dc.subjectneurotoxins
dc.subjectSecreted Phospholipases A(2)
dc.subjectPerfused Rat Kidney
dc.subjectPaw Edema
dc.subjectDiversity
dc.titleStructural and biological characterization of a crotapotin isoform isolated from Crotalus durissus cascavella venom
dc.typeArtículos de revistas


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