Artículos de revistas
Screening of yeast strains for transfructosylating activity
Registro en:
Journal Of Molecular Catalysis B-enzymatic. Elsevier Science Bv, v. 49, n. 41730, n. 43, n. 49, 2007.
1381-1177
WOS:000251158300008
10.1016/j.molcatb.2007.08.001
Autor
Maugeri, F
Hernalsteens, S
Institución
Resumen
Fructooligosaccharides (FOSs) are functional food ingredients with prebiotic properties, and a recent increase in the use of oligosaccharides in the food industry has led to the search for '' new '' microorganisms and enzymes for the production of oligosaccharides. This paper focuses on the screening of yeasts obtained from fruits and flowers (from Brazilian tropical forests), and capable of secreting extra-cellular enzymes with high fructosyl transferase activity (FTA). The screening and isolation procedures resulted in four potentially interesting yeast strains: Candida sp. (LEB-13), Rhodotorula sp. (LEB-U5.), Cryptococcus sp. (LEB-V2) and Rhodotorula sp. LEB-V10. All were able to produce more then 100 g l(-1) of FOS from a 500 g l(-1) sucrose solution, but only the last one, (LEB-V10), showed no hydrolytic activity with respect to the FOS produced, giving a continuous increase in FOS content up to the end of the reaction, when it was about 50% of the total carbohydrates. (c) 2007 Elsevier B.V. All rights reserved. 49 41730 43 49