Primary sequence determination of a Kunitz inhibitor isolated from Delonix regia seeds

dc.creatorPando, SC
dc.creatorOliva, MLV
dc.creatorSampaio, CAM
dc.creatorDi Ciero, L
dc.creatorNovello, JC
dc.creatorMarangoni, S
dc.date2001
dc.dateJUL
dc.date2014-11-15T00:22:15Z
dc.date2015-11-26T16:09:10Z
dc.date2014-11-15T00:22:15Z
dc.date2015-11-26T16:09:10Z
dc.date.accessioned2018-03-28T22:57:45Z
dc.date.available2018-03-28T22:57:45Z
dc.identifierPhytochemistry. Pergamon-elsevier Science Ltd, v. 57, n. 5, n. 625, n. 631, 2001.
dc.identifier0031-9422
dc.identifierWOS:000169481100002
dc.identifier10.1016/S0031-9422(01)00080-2
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/70286
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/70286
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/70286
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1266551
dc.descriptionA serine proteinase inhibitor was purified from Delonix regia seeds a Leguminosae tree of the Caesalpinioideae subfamily. The inhibitor named DrTI, inactivated trypsin and human plasma kallikrein with K-i values 2.19x10(-8) M and 5.25 nM, respectively. Its analysis by SDS-PAGE 10-20% showed that the inhibitor is a protein with a single polypeptide chain of M-r 22 h Da. The primary sequence of the inhibitor was determined by Edman degradation, thus indicating that it contained 185 amino acids and showed that it belongs to the Kunitz type family; however, its reactive site did not contain Arg or Lys at the putative reactive site (position 63, SbTI numbering) or it was displaced when compared to other Kunitz-type inhibitors. (C) 2001 Elsevier Science Ltd. All rights reserved.
dc.description57
dc.description5
dc.description625
dc.description631
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationPhytochemistry
dc.relationPhytochemistry
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectDelonix regia
dc.subjectLeguminosae
dc.subjectKunitz inhibitor
dc.subjectserine proteinase
dc.subjecttrypsin
dc.subjecthuman plasma kallikrein
dc.subjectAmino-acid-sequence
dc.subjectTrypsin-inhibitor
dc.subjectProteinase-inhibitors
dc.subjectChymotrypsin-inhibitor
dc.subjectBlood-coagulation
dc.subjectPurification
dc.subjectKallikrein
dc.subjectLegume
dc.titlePrimary sequence determination of a Kunitz inhibitor isolated from Delonix regia seeds
dc.typeArtículos de revistas


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