Artículos de revistas
Precipitation Of Porcine Insulin With Carbon Dioxide
Registro en:
Biotechnology And Bioengineering. , v. 103, n. 5, p. 909 - 919, 2009.
63592
10.1002/bit.22319
2-s2.0-67650819012
Autor
Tashima A.K.
Ottens M.
Van Der Wielen L.A.M.
Cintra D.E.
Pauli J.R.
De Alcantara Pessoa Filho P.
Miranda E.A.
Institución
Resumen
Recent works have pointed to the use of volatile electrolytes such as carbon dioxide (CO2) dissolved in aqueous solutions as a promising alternative to the precipitating agents conventionally used for protein recovery in the food and pharmaceutical industries. In this work we investigated experimental and theoretical aspects of the precipitation of porcine insulin, a biomolecule of pharmaceutical interest, using CO2 as an acid-precipitating agent. The solubility of porcine insulin in NaHCO3 solutions in pressurized CO2 was determined as a function of temperature and pressure, with a minimum being observed close to the protein isoelectric point. A thermodynamic model was developed and successfully utilized to correlate the experimental data. Insulin was considered a polyelectrolyte in the model and its self-association reactions were also taken into account. The biological activity of insulin was maintained after precipitation with CO 2, although some activity can be lost if foam is formed in the depressurization step. © 2009 Wiley Periodicals, Inc. 103 5 909 919 Asherie, N., Protein crystallization and phase diagrams (2004) Methods, 34, pp. 266-272 Bernardo, A., Calmanovici, C.E., Miranda, E.A., Induction time as an instrument to enhance comprehension of protein crystallization (2004) Crystal Growth and Design, 4 (4), pp. 799-805. , DOI 10.1021/cg034170+ Beutier, D., Renon, H., Representation of NH3-H2S-H2O, NH 3-CO2-H2O and NH3-SO 2-H2O vapor-liquid equilibria (1978) Ind Eng Chem Proc DD, 17, pp. 220-230 Blundell, T., Dodson, G., Hodgkin, D., Mercola, D., Insulin: The structure in the crystal and its reflection in chemistry and biology (1972) Advances in Protein Chemistry, pp. 279-403. , Anfinsen CB, Jr., Edsall JT, Richards FM, editors. New York: Academic Press Bonora, E., Manicardi, V., Zavaroni, I., Relationships between insulin secretion, insulin metabolism and insulin resistance in mild glucose intolerance (1987) Diabete et Metabolisme, 13 (2), pp. 116-121 Christopher, G.K., Phipps, A.G., Gray, R.J., Temperature-dependent solubility of selected proteins (1998) J Cryst Growth, 191, pp. 820-826 Clarkson, J.R., Cui, Z.F., Darton, R.C., Protein denaturation in foam. I. Mechanism study (1999) J Colloid Interf Sci, 215, pp. 323-332 Edsall, J.T., Gutfreund, H., (1983) Biothermodynamics - the Study of Biochemical Processes at Equilibrium, p. 248. , Chichester: John Wiley & Sons Edwards, T.J., Newman, J., Prausnitz, J.M., Thermodynamics of aqueous solutions containing volatile weak electrolytes (1975) AIChE J, 21, pp. 248-258 Edwards, T.J., Maurer, G., Newman, J., Prausnitz, J.M., VAPOR-LIQUID EQUILIBRIA in MULTICOMPONENT AQUEOUS SOLUTIONS of VOLATILE WEAK ELECTROLYTES (1978) AIChE J, 24 (6), pp. 966-976 Feeling-Taylor, A.R., Banish, R.M., Hirsch, R.E., Vekilov, P.G., Miniaturized scintillation technique for protein solubility determinations (1999) Rev Sci Instrum, 70, pp. 2845-2849 Feher, G., Kam, Z., Nucleation and growth of protein crystals: General principles and assays (1985) Method Enzymol, 114, pp. 77-112 Gehle, D., Schügerl, E., Protein recovery by continuous flotation (1984) Appl Microbiol Biotechnol, 20, pp. 133-138 Goldman, J., Carpenter, F.H., Zinc binding, circular dichroism, and equilibriumsedimentation studies on insulin (bovine) and several of its derivatives (1974) Biochemistry-US, 13, pp. 4566-4574 Golubovic, M., Van Hateren, S.H., Ottens, M., Witkamp, G.-J., Van Der Wielen, L.A.M., Novel method for the production of pure glycinin from soybeans (2005) Journal of Agricultural and Food Chemistry, 53 (13), pp. 5265-5269. , DOI 10.1021/jf0478206 Golubovic, M., Van Hateren, S.H., Ottens, M., Witkamp, G.J., Van Der Wielen, L.A.M., A method for lipase co-precipitation in a biodegradable protein matrix (2007) Biotechnology and Bioengineering, 98 (6), pp. 1209-1218. , DOI 10.1002/bit.21499 Goeppert, U., Maurer, G., VAPOR-LIQUID EQUILIBRIA in AQUEOUS SOLUTIONS of AMMONIA and CARBON DIOXIDE at TEMPERATURES between 333 and 393 K and PRESSURES UP to 7 MPa (1988) Fluid Phase Equilibria, 41 (1-2), pp. 153-185. , DOI 10.1016/0378-3812(88)80042-6 Helmerhorst, E., Stokes, G.B., The self-association of insulin: Determinations based on a differential gel adsorption procedure (1986) Archives of Biochemistry and Biophysics, 245 (1), pp. 238-247 Hofland, G.W., Van Es, M., Van Der Wielen, L.A.M., Witkamp, G.J., Isoelectric precipitation of casein using high pressure CO2 (1999) Ind Eng Chem Res, 38, pp. 4919-4927 Hofland, G.W., De Rijke, A., Thiering, R., Van Der Wielen, L.A.M., Witkamp, G.-J., Isoelectric precipitation of soybean protein using carbon dioxide as a volatile acid (2000) Journal of Chromatography B: Biomedical Sciences and Applications, 743 (1-2), pp. 357-368. , DOI 10.1016/S0378-4347(00)00259-0, PII S0378434700002590 Jeffrey, P.D., Coates, J.H., An equilibrium ultracentrifuge study of the self-association of bovine insulin (1966) Biochemistry-US, 5, pp. 489-498 Jordan, P.J., Lay, K., Ngan, N., Rodley, G.F., Casein production using high pressure carbon dioxide (1987) New Zeal J Dairy Sci, 22, pp. 247-256 Kaarsholm, N.C., Havelung, S., Hougaard, P., Ionization behavior of native and mutant insulins: PK perturbation of B13-Glu in aggregated species (1990) Arch Biochem Biophys, 283, pp. 496-502 Kawazuishi, K., Prausnitz John, M., CORRELATION of VAPOR-LIQUID EQUILIBRIA for the SYSTEM AMMONIA-CARBON DIOXIDE-WATER (1987) Industrial and Engineering Chemistry Research, 26 (7), pp. 1482-1485 Kurz, F., Rumpf, B., Maurer, G., Vapor-liquid-solid equilibria in the system NH3-CO 2-H2O from around 310 to 470 K: New experimental data and modeling (1995) Fluid Phase Equilibr, 104, pp. 261-275 Lundbaek, K., Intravenous glucose tolerance as a tool in definition and diagnosis of diabetes mellitus (1962) Br Med J, 1, pp. 1507-1513 Maa, Y.F., Hsu, C.C., Protein denaturation by combined effect of shear and air-liquid interface (1996) Biotechnol Bioeng, 54, pp. 503-512 Meyssami, B., Balaban, M.O., Teixeira, A.A., Prediction of pH in model systems pressurized with carbon dioxide (1992) Biotechnol Progr, 8, pp. 149-154 Milthorpe, B.K., Nichol, L.W., Jeffrey, P.D., The polymerization pattern of zinc(II)-insulin at pH 7.0 (1977) Biochim Biophys Acta, 495, pp. 195-202 Nettleton, E.J., Tito, P., Sunde, M., Bouchard, M., Dobson, C.M., Robinson, C.V., Characterization of the oligomeric states of insulin in self-assembly and amyloid fibril formation by mass spectrometry (2000) Biophysical Journal, 79 (2), pp. 1053-1065 Pessoa Filho, P.A., Mohamed, R.S., A chemical theory based equation of state for self-associating compounds (1999) Thermochimica Acta, 328 (1-2), pp. 65-71. , PII S004060319800625X Pitzer, K.S., Thermodynamics of electrolytes. I. Theoretical basis and general equations (1973) J Phys Chem, 77, pp. 268-277 Prausnitz, J.M., Lichtenthaler, R.N., Azevedo, E.G., (1999) Molecular Thermodynamics of Fluid-phase Equilibria, 3rd Edn, p. 864. , New Jersey: Prentice Hall Rumpf, B., Maurer, G., An experimental and theoretical investigation on the solubility of carbon dioxide in aqueous solutions of strong electrolytes (1993) Berich Bunsen Gesell, 97, pp. 85-97 Rumpf, B., Maurer, G., Solubility of carbon dioxide in aqueous solutions of ammonium chloride at temperatures from 313 K to 433 K and pressures up to 10 MPa (1994) Berich Bunsen Gesell, 98, pp. 1077-1081 Shih, Y.C., Prausnitz, J.M., Blanch, H.W., Some characteristics of protein precipitation by salts (1992) Biotechnol Bioeng, 40, pp. 1155-1164 Spilimbergo, S., Bertucco, A., Basso, G., Bertoloni, G., Determination of extracellular and intracellular pH of Bacillus subtilis suspension under CO2 treatment (2005) Biotechnol Bioeng, 92, pp. 447-451 Stryer, L., (1995) Biochemistry, 4th Edn, p. 1064. , New York: W.H. Freeman and Company Thiering, R., Hofland, G., Foster, N., Witkamp, G.-J., Van De Wielen, L., Carbon dioxide induced soybean protein precipitation: Protein fractionation, particle aggregation, and continuous operation (2001) Biotechnology Progress, 17 (3), pp. 513-521. , DOI 10.1021/bp010019l Thiering, R., Hofland, G., Foster, N., Witkamp, G.-J., Van De Wielen, L., Fractionation of soybean proteins with pressurized carbon dioxide as a volatile electrolyte (2001) Biotechnology and Bioengineering, 73 (1), pp. 1-11. , DOI 10.1002/1097-0290(20010405)73:1<1::AID-BIT1031>3.0.CO;2-B Tomasula, P.M., Craig, J.C., Boswell, R.T., Cook, R.D., Kuranz, M.J., Maxwell, M., Preparation of casein using carbon dioxide (1995) J Dairy Sci, 78, pp. 506-514 Tomasula, P.M., Craig, J.C., Boswell, R.T., A continuous process for casein production using high-pressure carbon dioxide (1997) Journal of Food Engineering, 33 (3-4), pp. 405-419. , PII S0260877497000538 Tomasula, P.M., Craig, J.C., Mcaloon, A.J., Economic analysis of a continuous casein process using carbon dioxide as precipitant (1998) J Dairy Sci, 81, pp. 3331-3342 Vekilov, P.G., Feeling-Taylor, A.R., Yau, S.-T., Petsev, D., Solvent entropy contribution to the free energy of protein crystallization (2002) Acta Crystallographica Section D: Biological Crystallography, 58 (10), pp. 1611-1616. , DOI 10.1107/S0907444902014312 Wintersteiner, O., Abramson, H.A., The isoelectric point of insulin-electrical properties of adsorbed and crystalline insulin (1933) J Biol Chem, 99, pp. 741-753 Xia, J., Rumpf, B., Maurer, G., Solubility of carbon dioxide in aqueous solutions containing sodium acetate or ammonium acetate at temperatures from 313 to 433 K and pressures up to 10 MPa (1999) Fluid Phase Equilibria, 155 (1), pp. 107-125. , DOI 10.1016/S0378-3812(98)00464-6, PII S0378381298004646 Xia, J., Perez-Salado Kamps, A., Rumpf, B., Maurer, G., Solubility of hydrogen sulfide in aqueous solutions of single strong electrolytes sodium nitrate, ammonium nitrate, and sodium hydroxide at temperatures from 313 to 393 K and total pressures up to 10 MPa (2000) Fluid Phase Equilibria, 167 (2), pp. 263-284. , DOI 10.1016/S0378-3812(99)00324-6, PII S0378381299003246