Actas de congresos
Random Amplified Polymorphic Dna Of Screwworm Fly Populations (diptera: Calliphoridae) From Southeastern Brazil And Northern Argentina
Registro en:
Genome. , v. 42, n. 4, p. 772 - 779, 1999.
8312796
2-s2.0-0033373974
Autor
Infante-Malachias M.E.
Yotoko K.S.C.
Lima De Azeredo Espin A.M.
Institución
Resumen
The screwworm fly Cochliomyia hominivorax is one of the most important agents of traumatic myiasis throughout neotropical regions. In this work, we optimized the technique of RAPD-PCR for these species and used it to study the genetic variability among seven populations (six from southeastern Brazil and one from northern Argentina) of C. hominivorax. RAPD fingerprints showed high variation for 12 primers used, revealing 209 presumptive loci of which 198 were polymorphic. Marker pattern relationships for these different populations were used to determine genetic relatedness, as well as to examine potential patterns of gene flow. Our interpretation of Lynch and Milligan's analogue of Wright's F((ST)) was that C. hominivorax populations are genetically subdivided (F'((ST)) for pooled samples = 0.122). Our data suggested that the subdivision detected in C. hominivorax populations by RAPD can be explained by the interplay of random factors affecting allele frequency changes. These results indicate that the RAPD-PCR technique is useful for revealing genetic variation in screwworm fly populations not detected by others techniques and can represent an efficient method for understanding the genetic structure and population genetic phenomena of this important pest. 42 4 772 779 Apostol, B.L., Black W.C. IV, Reiter, P., Miller, B., Population genetics with RAPD-PCR markers: The breeding structure of Aedes aegypti in Puerto Rico (1996) Heredity, 76, pp. 325-334 Baruffi, L., Damiani, G., Guglielmino, C.R., Bandis, C., Malacrida, A.R., Gasperi, G., Polymorphism within and between populations of Ceratitis capitata: Comparison betwee RAPD and multilocus enzyme electrophoresis data (1995) Heredity, 74, pp. 425-437 Begun, D.J., Aquadro, M., Levels of naturally occurring DNA polymorphism are correlated with recombination rates in Drosophila melanogaster (1992) Nature (London), 356, pp. 519-520 Bielawsky, J.P., Pumo, D.E., Randomly amplified polymorphic DNA analysis of atlantic coast striped bass (1997) Heredity, 78, pp. 32-40 Black W.C. IV, Duteau, N.M., Puterka, G.J., Nichols, J.R., Pettorini, J.M., Use of the random amplified polymorphic dna polymerase chain reaction (RAPD-PCR) to detect DNA polymorphism in Aphids (Homóptera: Aphididae) (1992) Bull. Entomol. Res., 82, pp. 151-159 Bush, G.L., Neck, R.W., Ecological genetics of the Screwworm fly, Cochliomyia hominivorax (Diptera: Calliphoridae) and its bearing on the quality control of mass reared insect (1976) Environ. Entomol., 5, pp. 821-826 Coquerel, C., Note sur une larve d'oestridae extraite du bras d'un homme à Cayenne (1859) Rev. Mag. Zool, 2 (11), p. 356 Crespi, B.J., Heterozigosity in haplodiploid thysanoptera (1991) Evolution, 45, pp. 458-464 Dear, J.P., A revision of the new world Chrysomyini (Diptera:Calliphoridae) (1985) Rev. Bras. Zool., 3, pp. 109-169 Dev, V., La Chance, L.E., Whitten, C.J., Polytene chromosomes, karyotype correlations and population cytology of the primary screwworm fly (1986) J. Hered., 77, pp. 427-434 Gibbs, H.L., Prior, K.A., Weatherhead, P.J., Genetic analysis of populations of threatened snake species using RAPD markers (1994) Mol. Ecol., 3, pp. 329-337 Graham, O.H., Symposium on eradication of the screwworm from the United States and Mexico (1985) Misc. Publ. Entomol. Soc. Am., 62, pp. 1-68 Graur, D., Gene diversity in Himenoptera (1985) Evolution, 39, pp. 190-199 Guimarães, J.H., Papavero, N., Pires Do Prado, A., As miíases na região Neotropical identificaçào, biologia, bibliografia (1983) Rev. Bras. Zool., 1 (4), pp. 239-416 Hadrys, H., Balick, M., Schierwater, B., Applications of random amplified polymorphic DNA (RAPD) in molecular ecology (1992) Mol. Ecol., 1, pp. 55-63 Hall, M., Wall, R., Myiasis of humans and domestic animals (1995) Adv. Parasitol., 35, pp. 57-334 Haymer, D.S., Random amplified polymorphic DNA and microsatellites. What are they and can they tell us anything we don't already know? (1994) Ann. Entomol. Soc. Am., 87, pp. 717-722 Haymer, D.S., Mei, H., McInnis, D.O., Genetic marker analysis of spatial and temporal relationships among existing populations and new infestations of the mediterranean fruit fly (Ceratitis capitata) (1997) Heredity, 79, pp. 302-309 Hedrick, P., Shooting the RAPD's (1992) Nature (London), 355, pp. 679-680 Hartl, D.L., (1988) A Primer of Population Genetics, , Sinauer Associates, Inc. Sunderland, Mass Infante, M.E., Azeredo Espin, A.M.L., Genetic variability in mitochondrial DNA of screwworm, Cochliomyia hominivorax (Diptera: Calliphoridae), from Brazil (1995) Biochem. Genet., 33, pp. 737-756 Innis, M.A., Gelfand, D.H., Optimization of PCR (1990) PCR Protocols: A Guide to Methods and Applications, pp. 3-12. , Edited by M.A. Innis, D.H. Gelfand, J.J. Sninsky, and T.J. White. Academic Press Inc., San Diego, Calif Krafsur, E.S., Whitten, C.J., Breeding structure of screwworm fly populations (Diptera:Calliphoridae) in Colima, Mexico (1993) J. Med. Entomol., 30, pp. 477-480 LaChance, E.E., Whitten, C.J., Cytogenetic studies of screwworm (Diptera:Calliphoridae) populations from Southern Mexico and Jamaica. Symposium: Genetics in entomology (1986) Ann. Entomol. Am., 79, p. 729 Lu, R., Rank, G.H., Use of RAPD analysis to estimate population genetic parameters in the alfalfa leaf cutting bee, Megachile rotundata (1996) Genome, 39, pp. 655-663 Lynch, M., Milligan, B.G., Analysis of population genetic structure with RAPD markers (1994) Mol. Ecol., 3, pp. 91-99 Nei, M., Genetic distance between populations (1972) Am. Nat., 106, pp. 283-292 Nei, M., (1987) Molecular Evolutionary Genetics, , Columbia University Press. New York Nei, M., Li, W.H., Mathemathical model for studying genetic variation in terms of restriction endonucleases (1979) Proc. Natl. Acad. Sci. U.S.A., 76, pp. 5269-5273 Novo, M.T.M., De Souza, A.P., Garcia O., Jr., Ottoboni, L.M.M., RAPD genomic fingerprinting differenciates Thiobacillus ferrooxidans strains (1996) Syst. Appl. Microbiol., 19, pp. 91-95 Plikaytis, B.D., Carlone, G.M., Edwards, P., Mayer, W., Robust estimation of standard curves for protein molecular weigth and linear-duplex DNA based-pair number after gel electrophoresis (1986) Anal. Biochem., 152, pp. 346-364 Pomonis, J.G., Cuticular hydrocarbons of the screwworm, Cochliomyia hominivorax (Diptera:Calliphoridae). Isolation, identification and quantification as a funtion of age, sex and irradiation (1989) J. Chem. Ecol., 15, p. 2301 Roehrdanz, R.L., Intraspecific genetic variability in mitochondrial DNA of screwworm fly (C.hominivorax) (1989) Biochem. Genet., 27, pp. 551-559 Roehrdanz, R.L., Johnson, D.A., Mitochondrial DNA variation among geographical populations of the Screwworm fly, Cochliomyia hominivorax (1988) J. Med. Entomol., 25 (2), pp. 136-141 Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning. A Laboratory Manual, , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y Stevens, J., Wall, R., The use of random amplified polymorphic DNA (RAPD) analysis for studies of genetic variation in populations of the blowfly Lucilia sericata (Diptera: Calliphoridae) in southern England (1995) Bull. Entomol. Res., 85 (4), pp. 549-555 Taylor, D.B., Petterson R.D., Jr., Population genetics and gene variation in primary and secondary screwworm (Diptera: Calliphoridae) (1994) Ann. Entomol. Soc. Am., 87, pp. 626-633 Taylor, D.B., Petterson R.D., Jr., Moya-Borja, G.E., Populations genetics and gene variation in screwworms (Diptera: Calliphoridae) from Brazil (1996) Biochem. Genet., 34 (1-2), pp. 67-76 Whitten, C.J., Use of the isozyme technique to asses the quality of mass-reared sterile screwworm flies (1980) Ann. Entomol. Soc. Am., 73, pp. 7-10 Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalsky, J.A., Tingey, S.V., DNA polymorphism amplified by arbitrary primers are useful as genetic markers (1990) Nucleic Acids. Res., 18, pp. 6531-6535 Welsh, J., McCleland, M., Fingerprint genomes using PCR with arbitrary primers (1990) Nucleic. Acids. Res., 18, pp. 7213-7218 Wright, S., (1978) Evolution and the Genetics of Populations. Vol.4. Variability within and among Natural Populations, 4. , University of Chicago Press. Chicago Yong, H.S., Low allozyme variability in Batrocera albistrigata (Insecta: Diptera: Tephritidae) from peninsular Malasya (1990) Comp. Biochem. Physiol. B Comp. Biochem. Mol. Biol., 97, pp. 119-121