Artículos de revistas
The Hemibiotrophic Cacao Pathogen Moniliophthora Perniciosa Depends On A Mitochondrial Alternative Oxidase For Biotrophic Development
Registro en:
New Phytologist. , v. 194, n. 4, p. 1025 - 1034, 2012.
0028646X
10.1111/j.1469-8137.2012.04119.x
2-s2.0-84860498447
Autor
Thomazella D.P.T.
Teixeira P.J.P.L.
Oliveira H.C.
Saviani E.E.
Rincones J.
Toni I.M.
Reis O.
Garcia O.
Meinhardt L.W.
Salgado I.
Pereira G.A.G.
Institución
Resumen
The tropical pathogen Moniliophthora perniciosa causes witches' broom disease in cacao. As a hemibiotrophic fungus, it initially colonizes the living host tissues (biotrophic phase), and later grows over the dead plant (necrotrophic phase). Little is known about the mechanisms that promote these distinct fungal phases or mediate the transition between them. An alternative oxidase gene (Mp-aox) was identified in the M. perniciosa genome and its expression was analyzed througout the fungal life cycle. In addition, the effects of inhibitors of the cytochrome-dependent respiratory chain (CRC) and alternative oxidase (AOX) were evaluated on the invitro development of M. perniciosa. Larger numbers of Mp-aox transcripts were observed in the biotrophic hyphae, which accordingly showed elevated sensitivity to AOX inhibitors. More importantly, the inhibition of CRC prevented the transition from the biotrophic to the necrotrophic phase, and the combined use of a CRC and AOX inhibitor completely halted fungal growth. On the basis of these results, a novel mechanism is presented in which AOX plays a role in the biotrophic development of M. perniciosa and regulates the transition to its necrotrophic stage. 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