Artículos de revistas
Endo-β-mannanase From The Endosperm Of Seeds Of Sesbania Virgata (cav.) Pers. (leguminosae): Purification, Characterisation And Its Dual Role In Germination And Early Seedling Growth
Registro en:
Brazilian Journal Of Plant Physiology. , v. 18, n. 2, p. 269 - 280, 2006.
16770420
2-s2.0-33749379881
Autor
Lisboa C.G.S.
Tonini P.P.
Tine M.A.S.
Buckeridge M.S.
Institución
Resumen
Galactomannans are storage cell wall polysaccharides present in seeds of some legumes. Their degradation is carried out by three hydrolases (α-galactosidase (EC 3.2.1.22), endo-β-mannanase (EC 3.2.1.78) and β-mannosidase (EC 3.2.1.25)). In the present study we purified and characterised an endo-β-mannanase from seeds of Sesbania virgata and addressed its role in germination and seedling development. The polypeptide purified by Ion Exchange Chromatography and Affinity Chromatography on Sepharose-Concanavalin A, showed a pH optimum between 3.5 and 5 at 45°C and high stability at pH 7.8. The low stability at pH 5 appears to be associated with isoelectric precipitation, in view of the pI of the enzyme being 4.5. The purified enzyme is a glycoprotein with a molecular mass of 26 KDa by SDS-PAGE and 36 KDa by gel chromatography. The purified polypeptide attacked galactomannan from different sources, being more effective on polymers with a lower degree of galactosylation (from carob gum), in comparison with medium or highly galactosylated galactomannans (from guar, S. virgata and fenugreek), respectively. A peak of endo-β-mannanase activity was detected during radicle protrusion in the endosperm tissue surrounding the radicle and later on in the lateral endosperm. This second peak was associated with the period of reserve mobilisation. Using an antibody raised against coffee endo-β-mannanase, the enzyme could be detected in immunodot-blots performed with extracts of S. virgata endosperms. The results are consistent with the hypothesis that the peak of endo-mannanase during germination facilitates radicle protrusion through the surrounding endosperm by weakening it in the region close to the radicle tip. 18 2 269 280 Bradford, M.M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254 Buckeridge, M.S., Dietrich, S.M.C., Galactomannan from Brazilian legume seeds (1990) Rev. Brasil. Bot., 13, pp. 109-112 Buckeridge, M.S., Dietrich, S.M.C., Mobilisation of the raffinose family oligosaccharides and galactomannan in germinating seeds of Sesbania marginata Benth (Leguminosae-Faboideae) (1996) Plant Sci., 117, pp. 33-43 Buckeridge, M.S., Dietrich, S.M.C., Lima, D.U., Galactomannans as the reserve carbohydrate of legume seeds (2000) Developments in Crop Science, 26, pp. 283-316. , Gupta A.K., Kaur N. (eds), Elsevier Science B. V., Amsterdam Buckeridge, M.S., Santos, H.P., Tiné, M.A.S., Mobilisation of storage cell wall polysaccharides in seeds (2000) Plant Physiol. Biochem., 38, pp. 141-156 Dea, I.C.M., Morrinson, A., Chemistry and interactions of seed galactomannans (1975) Adv. Charbohydr. Chem. Biochem., 31, pp. 241-312 Dion, A.S., Pometi, A.A., Ammoniacal silver staining of proteins: Mechanisms of glutaraldehyde enhancement (1983) Anal. Biochem., 129, pp. 490-496 Downie, B., Hilhorst, H.W.M., Bewley, J.D., A new assay for quantifying endo-β-mannanase activity using Congo Red dye (1994) Phytochemistry, 36, pp. 829-835 Dulson, J., Bewley, J.D., Johnson, R.H., Abscisic acid is an endogenous inhibitor in the regulation of mannanase production by isolated lettuce endosperms (1988) Plant Physiol., 87, pp. 660-665 Groot, S.P.C., Karssen, C.M., Gibberellins regulate seed-germination in tomato by endosperm weakening: A study with gibberellin-deficient mutants (1987) Planta, 171, pp. 525-531 Groot, S.P.C., Karssen, C.M., Dormancy and germination of abscisic acid-deficient tomato seeds: Studies with the sitiens mutant (1992) Plant Physiol., 99, pp. 952-958 Halmer, P., Bewley, J.D., Mannanase production by the lettuce endosperm: Control by the embryo (1979) Planta, 144, pp. 333-340 Kontos, F., Spyropoulos, C.G., Production and secretion of alpha-galactosidase and endo-β-mannanase activity by carob (Ceratonia siliqua L.) in the endosperm protoplast (1995) J. Exp. Bot., 46, pp. 577-583 Laemmli, U.K., Cleavage of the structural protein during the assembly of the head of bacteriophage T4 (1970) Nature, 223, pp. 680-685 McCleary, B.V., Modes of action of β-D-mannanase enzymes of diverse origin on legume seed galactomannans (1979) Phytochemistry, 18, pp. 757-763 McCleary, B.V., Enzymic interactions in the hydrolysis of galactomannan in germinating guar: The role of exo-β-mannanase (1983) Phytochemistry, 22, pp. 649-658 McCleary, B.V., Matheson, N.K., Galactomannan structure and β-mannanase and β-mannosidase activity in germinating legume seeds (1975) Phytochemistry, 14, pp. 1187-1194 Meier, H., Reid, J.S.G., Morphological aspects of galactomannan formation in the endosperm of Trigonella foenumgraecum L. (Leguminosae) (1977) Planta, 133, pp. 243-248 Mo, B., Bewley, J.D., β-mannosidase (Ec 3.2.1.25) activity during and following germination of tomato (Lycopersicon esculentum Mill.) seeds: Purification, cloning and characterisation (2002) Planta, 215, pp. 141-152 Nonogaki, H., Gee, O.H., Bradford, K.J., A germination-specific endo-β-mannanase gene is expressed in the micropylar endosperm cap of tomato seeds (2000) Plant Physiol., 123, pp. 1235-1245 Nonogaki, H., Matsushima, H., Morohashi, Y., Galactomannan hydrolysing activity develops during priming in the micropylar endosperm tip of tomato seeds (1992) Plant Physiol., 110, pp. 167-172 Potomati, A., Buckeridge, M.S., Effect of abscisic acid on the mobilisation of galactomannan and embryo development of Sesbania virgata (Cav.) Pers. (Leguminosae-Faboideae) (2002) Rev. Brasil. Bot., 25, pp. 303-310 Reid, J.S.G., Reserve carbohydrate metabolism in germinating seeds of Trigonella foenun-graecum L. (Legum) (1971) Planta, 100, pp. 131-142 Reid, J.S.G., Cell wall storage carbohydrates in seeds: Biochemistry of the seeds gums and hemicelluloses (1985) Adv. Bot. Res., 11, pp. 125-155 Reid, J.S.G., Davies, C., Endo-β-mannanase, the Leguminous aleurone layer and storage galactomannan in germinating seeds of Trigonella foenum-graecum L. (1977) Planta, 133, pp. 219-222 Reid, J.S.G., Meier, H., Chemotaxonomic aspects of the reserve galactomannan in leguminous seeds (1970) Z. Pflanzenphysiol., 62, pp. 89-92 Reid, J.S.G., Meier, H., The function of the aleurone layer during galactomannan mobilisation in germinating seeds fenugreek (Trigonella foenum-graecum L.), crimson clover (Trigonella incarnatum L.) and lucerne (Medicago sativa L.): A correlative biochemical and ultrastructural study (1972) Planta, 106, pp. 44-60 Reid, J.S.G., Meier, H., Enzyme activities and galactomannan mobilisation in germinating seeds, of fenugreek (Trigonella foenum-graecum L. Leguminosae): Secretion of α-galactosidases and β-mannosidases by aleurone layer (1973) Planta, 112, pp. 301-308 Sioufi, A., Percheron, F., Courtois, J.E., Nucleoside-diphosphateoses et metabolism glucidique au cours de la germination chez le fenugrec (1970) Phytochemistry, 9, pp. 991-999 Spyropoulos, C.G., Reid, J.S.G., Regulation of α-galactosidase activity and the hydrolysis of galactomannan in the endosperm of the fenugreek (Trigonella foenum-graecum) seed (1985) Planta, 166, pp. 271-275 Spyropoulos, C.G., Reid, J.S.G., Water stress and galactomannan breakdown in germinated fenugreek seeds: Stress affects the production and activities in vivo of galactomannan hydrolysing enzymes (1988) Planta, 179, pp. 403-408 Still, D.W., Bradford, K.J., Endo-β-mannanase activity from individual tomato endosperm caps and radicle tips in relation to germination rates (1997) Plant Physiol., 113, pp. 21-29 Tiné, M.A.S., Lima, D.U., Buckeridge, M.S., Galactose branching modulates the action of cellulase on seed storage xyloglucans (2003) Carbohydr. Polym., 52, pp. 135-141 Toorop, P.E., Bewley, J.D., Hilhorst, H.W.M., Endo-β-mannanase isoforms are present in the endosperm and embryo tomato seeds, but not essentially linked to the completion of germination (1996) Planta, 200, pp. 153-158