Analysis Of The Ergosterol Biosynthesis Pathway Cloning, Molecular Characterization And Phylogeny Of Lanosterol 14 α-demethylase (erg11) Gene Of Moniliophthora Perniciosa

dc.creatorCeita G.O.
dc.creatorCeita G.O.
dc.creatorVilas-Boas L.A.
dc.creatorCastilho M.S.
dc.creatorCarazzolle M.F.
dc.creatorPirovani C.P.
dc.creatorSelbach-Schnadelbach A.
dc.creatorGramacho K.P.
dc.creatorRamos P.I.P.
dc.creatorBarbosa L.V.
dc.creatorPereira G.A.G.
dc.creatorGoes-Neto A.
dc.date2014
dc.date2015-06-25T17:52:02Z
dc.date2015-11-26T14:12:46Z
dc.date2015-06-25T17:52:02Z
dc.date2015-11-26T14:12:46Z
dc.date.accessioned2018-03-28T21:13:27Z
dc.date.available2018-03-28T21:13:27Z
dc.identifier
dc.identifierGenetics And Molecular Biology. Brazilian Journal Of Genetics, v. 37, n. 4, p. 683 - 693, 2014.
dc.identifier14154757
dc.identifier
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84914703975&partnerID=40&md5=fa8add754f026cff86da645b689902d8
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/86196
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/86196
dc.identifier2-s2.0-84914703975
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1242097
dc.descriptionThe phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches’ broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed.ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. perniciosa enzyme was most closely related to that of Coprinopsis cinerea.
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dc.languageen
dc.publisherBrazilian Journal of Genetics
dc.relationGenetics and Molecular Biology
dc.rightsaberto
dc.sourceScopus
dc.titleAnalysis Of The Ergosterol Biosynthesis Pathway Cloning, Molecular Characterization And Phylogeny Of Lanosterol 14 α-demethylase (erg11) Gene Of Moniliophthora Perniciosa
dc.typeArtículos de revistas


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