Artículos de revistas
Forced-swim Induces Subsensitivity To Phenylephrine In The Rat Thoracic Aorta [a Natação Forçada Induz Subsensibilidade à Fenilefrina Em Aorta Torácica De Rato]
Registro en:
Revista Brasileira De Ciencias Farmaceuticas/brazilian Journal Of Pharmaceutical Sciences. , v. 39, n. 4, p. 433 - 439, 2003.
15169332
2-s2.0-1842428712
Autor
Sampaio Moura M.J.C.
De Quintal M.M.
Marcondes F.K.
Institución
Resumen
Stress may change vascular function. The aim of this report was to study the sensitivity to phenylephrine (PHE) in the thoracic aorta from rats submitted to forced-swim. Male Wistar rats (200-250 g) were submitted to three swimming sessions, one session/day (15, 30 and 30 min, respectively). Immediately after the last swimming session, the animals were sacrificed and thoracic aorta was isolated. Aortic rings (3-5 mm), with and without endothelium, were carefully obtained and were maintained in Krebs-Henseleit solution (95% O 2 - 5% CO 2, 37 °C). Endothelial integrity was assessed by relaxation to acetylcholine (10 μM) in pre-contracted rings (PHE 0.1 μM). Concentration-effect curves to PHE were obtained (n = 5/group). There was no difference between control and stress groups in the maximum response to PHE of aortic rings with and without endothelium (p>0.05). Forced-swim induced subsensitivity to PHE in aortic rings with endothelium isolated from stressed rats (pD 2 = 6.89 ± 0.07; p<0.05) compared to the control group (pD 2 = 7.39 ± 0.06), without changes in aortic rings without endothelium. The in vitro inhibition of nitric oxide synthesis cancelled this subsensitivity. It is concluded that forced swim-induced-subsensitivity to PHE in thoracic aorta from rats seems to be caused by an increase in the activity of the endothelial nitric oxide system. 39 4 433 439 Bassani, R.A., De Moraes, S., Subsensitivity to beta adrenoceptor agonists in right atria isolated from footshock stressed rats (1987) Gen. Pharmacol., Oxford, 18 (5), pp. 473-477 Bianchi, F.J., Tanno, A.P., Marcondes, F.K., Relação entre nivel de estresse e supersensibilidade à norepinefrina em ratas no proestro (2001) Rev. Bras. Ciênc. Farm., São Paulo, 37 (3), pp. 391-398 Callia, M.L., De Moraes, S., Heterogeneity of beta adrenoceptors in right atria isolated from cold-exposed rats (1984) J. Pharmacol. Exp. Ther., Baltimore, 230 (2), pp. 450-454 Cannon, W.B., Querido, S., Britton, S.W., Bright, E.M., Studies on the conditions of activity in endocrine glands. The role of adrenal excretion in the chemical control of body temperature (1927) Am. J. Physiol., Bethesda, 79, pp. 466-506 Capaz, F.R., De Moraes, S., Reduction by acute restraint stress of norepinephrine sensitivity in the isolated rat pacemaker (1988) Eur. J. Pharmacol., Amsterdam, 147 (2), pp. 295-298 Cordellini, S., Vassilieff, V.S., Decreased endothelium-dependent vasoconstriction to noradrenaline in acute-stressed rats is potentiated by previous chronic stress: Nitric oxide involvement (1998) Gen. Pharmacol., Oxford, 30, pp. 79-83 Delp, M.D., McAllister, R.M., Laughlin, M.H., Exercise training alters endothelium-dependent vasoreactivity of rat abdominal aorta (1993) J. Appl. Physiol., Bethesda, 75, pp. 1354-1363 De Moraes, S., Carvalho, A.J., Cavalcante, M.T., Mathias, S.R., Relative hypoxia-induced contraction of the isolated human umbilical artery (1995) Pharmacol. & Toxicol., Copenhagen, 76, pp. 218-220 Edwards, J.G., Tipton, C.M., Matthes, R.D., Influence of exercise training on reactivity and contractility of arterial strips from hypertensive rats (1985) J. Appl. Physiol., Bethesda, 58, pp. 1683-1688 Feletou, M.W., Vanhoutte, P.M., Endothelium-dependent hyperpolarization of canine coronary smooth muscle (1976) Br. J. Pharmacol., London, 93, pp. 515-524 Flain, S.F., Hsieh, A.C.L., Effect of cold-acclimation on rabbit carotid artery: Altered response to norepinephrine (1978) Gen. Pharmacol., Oxford, 78, pp. 437-442 Furchgott, R.E., Zawadzki, J.V., The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine (1980) Nature, London, 288, pp. 373-376 Garcia-Marquez, C.G., Armario, A., Chronic stress depresses exploratory activity and behavioral performance in the forced swimming test without altering ACTH response to a novel acute stressor (1987) Physiol. Behav., New York, 40, pp. 33-38 Gryglewski, R.J., Botting, R.M., Vane, J.R., Mediators produced by endothelial cells (1988) Hypertension, Philadelphia, 12, pp. 530-548 Harri, M.N.E., Melender, L., Tirri, R., Changed chronotropic sensitivity to sympathomimetic amines in isolated atria from rats following cold acclimation (1974) Experientia, Basel, 30 (9), pp. 1041-1043 Jansakul, C., Effect of swimming on vascular reactivity to phenylephrine and KCl in male rats (1995) Br. J. Pharmacol., London, 115, pp. 587-594 Lamb, V.L., Schwartz, A., Rohn, W.R., Kaiser, L., Ciclooxigenase inhibitors depress norepinephrine constriction of rat abdominal, but not thoracic, aorta (1994) Eur. J. Pharmacol., Amsterdam, 256, pp. 221-226 Marcondes, F.K., Vanderlei, L.C.M., Lanza, L.L.B., Spadari-Bratfisch, R.C., Stress-induced subsensitivity to catecholamines depends on the estrous cycle. Can (1996) J. Physiol. Pharmacol., Ottawa, 74 (6), pp. 663-669 Martins, M.C., Spadari, R.C., Stress-induced desensitization of the cardiovascular response to noradrenaline in unanesthetized rats (1990) Braz. J. Med. Biol. Res., São Paulo, 23, pp. 1041-1044 McEwen, B.S., Definitions and Concepts of Stress (2000) Encyclopedia of Stress, 3, pp. 508-509. , Fink, G., ed. USA: Academic Press Moncada, S., Palmer, R.M.J., Higgs, E.A., Nitric oxide: Physiology, pathology and pharmacology (1991) Pharmacol. Rev., Bethesda, 43, pp. 109-142 Moura, M.J.C.S., De Moraes, S., Forced swim stress: Supersensitivity of the isolated rat pacemaker to the chronotropic effect of isoprenaline and the role of corticosterone (1994) Gen. Pharmacol., Oxford, 25, pp. 1341-1347 Moura, M.J.C.S., Marcondes, F.K., Influence of estradiol and progesterone on the sensitivity of rat thoracic aorta to noradrenaline (2001) Life Sci., Oxford, 68 (8), pp. 881-888 Navarro-Oliveira, C.M., Vassilieff, V.S., Cordellini, S., The sympathetic adrenomedullary system, but not the hypothalamic-pituitary-adrenal axis, participates in aorta adaptive response to stress: Nitric oxide involvement (2000) Auton. Neurosci., Amsterdam, 83 (3), pp. 140-147 Nourani, F.R.R., Spadari, R.C., De Moraes, S., Footshock stress-induced supersensitivity to isoprenaline in the isolated pacemaker of the rat: Effects of the compounds RU-38486 and RU-28362 (1992) Gen. Pharmacol., Oxford, 23, pp. 787-791 Palmer, R.M.J., Ferridge, A.C., Moncada, S., Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor (1987) Nature, London, 327, pp. 524-526 Oltman, C.L., Parker, J.L., Adams, H.R., Laughlin, M.H., Effects of exercise training on vasomotor reactivity of porcine coronary arteries (1992) Am. J. Physiol., Bethesda, 263, pp. H372-H382 Östman-Smith, I., Adaptive changes in the sympathetic nervous system and some effector organs of the rat following long-term exercise or cold acclimation and the role of cardiac sympathetic nerves in the genesis of compensatory cardiac hypertrophy (1979) Acta Physiol. Scand., Oxford, (SUPPL. 477), pp. 1-118 Plante, G.E., Vascular response to stress in health and disease (2002) Metabolism, Bethesda, 51 (6 SUPPL. 1), pp. 25-30 Porsolt, R.D., Anton, G., Blavet, N., Jalfre, M., Behavioral despair in rats: A new model sensitive to antidepressant treatments (1978) Eur. J. Pharmacol., Amsterdam, 47, pp. 379-391 Rogers, P.J., Miller, T.D., Bauer, B.A., Brum, J.M., Bove, A.A., Vanhoutte, P.M., Exercise training and responsiveness of isolated coronary arteries (1991) J. Appl. Physiol., Bethesda, 71, pp. 2346-2351 Rubanyi, G.M., Vanhoutte, P.N., Hypoxia releases a vasoconstrictor substance from canine vascular endothelium (1985) J. Physiol., London, 363, pp. 45-56 Selye, H., A syndrome produced by diverse nocuous agents (1936) Nature, London, 138 (1), p. 32 Spadari, R.C., De Moraes, S., Repeated swimming stress and responsiveness of isolated rat pacemaker to chronotropic effect of noradrenaline and isoprenaline: Role of adrenal corticosteroids (1988) Gen. Pharmacol., Oxford, 19 (4), pp. 553-557 Spadari, R.C., Bassani, R.A., De Moraes, S., Supersensitivity to isoprenaline and epinephrine in right atria isolated from rats submitted to a single swimming session (1988) Gen. Pharmacol., Oxford, 19 (1), pp. 129-135 Spier, S.A., Laughlin, M.H., Delp, M.D., Effects of acute and chronic exercise on vasoconstrictor responsiveness of rat abdominal aorta (1999) J. Appl. Physiol., Bethesda, 87, pp. 1752-1757 Tanno, A.P., Bianchi, F.J., Marcondes, F.K., Influência do ciclo estral sobre a sensibilidade da resposta cronotrópica à norepinefrina em ratas submetidas a estresse agudo (2002) Rev. Bras. Ciênc. Farm., São Paulo, 38 (1), pp. 63-70 Tanno, A.P., Bianchi, F.J., Moura, M.J.C.S., Marcondes, F.K., Atrial supersensitivity to noradrenaline in stressed female rats (2002) Life Sci., Oxford, 71 (25), pp. 2973-2981 Van Rossum, J.M., Cumulative dose-response curves. II. Technique for the making of dose-response curves in isolated organs and the evaluation of the drug parameters (1963) Arch. Int. Pharmacodyn. Ther., Ghent, 143, pp. 229-230 Yanagisawa, M., Kurihara, H., Kimura, S., Tomobe, Y., Kobayashi, M., Mitsue, Y., Yasaki, Y., Masaki, T., A novel potent vasoconstrictor peptide produced by vascular endothelial cells (1988) Nature, London, 332, pp. 411-415