Actas de congresos
Drug Loading To Lipid-based Cationic Nanoparticles
Registro en:
Nuclear Instruments And Methods In Physics Research, Section B: Beam Interactions With Materials And Atoms. , v. 238, n. 1-4, p. 290 - 293, 2005.
0168583X
10.1016/j.nimb.2005.06.065
2-s2.0-25144449139
Autor
Cavalcanti L.P.
Konovalov O.
Torriani I.L.
Haas H.
Institución
Resumen
Lipid-based cationic nanoparticles are a new promising option for tumor therapy, because they display enhanced binding and uptake at the neo-angiogenic endothelial cells, which a tumor needs for its nutrition and growth. By loading suitable cytotoxic compounds to the cationic carrier, the tumor endothelial and consequently also the tumor itself can be destroyed. For the development of such novel anti-tumor agents, the control of drug loading and drug release from the carrier matrix is essential. We have studied the incorporation of the hydrophobic anti-cancer agent Paclitaxel (PXL) into a variety of lipid matrices by X-Ray reflectivity measurements. Liposome suspensions from cationic and zwitterionic lipids, comprising different molar fractions of Paclitaxel, were deposited on planar glass substrates. After drying at controlled humidity, well ordered, oriented multilayer stacks were obtained, as proven by the presence of bilayer Bragg peaks to several orders in the reflectivity curves. The presence of the drug induced a decrease of the lipid bilayer spacing, and with an excess of drug, also Bragg peaks of drug crystals could be observed. From the results, insight into the solubility of Paclitaxel in the model membranes was obtained and a structural model of the organization of the drug in the membrane was derived. Results from subsequent pressure/area-isotherm and grazing incidence diffraction (GID) measurements performed with drug/lipid Langmuir monolayers were in accordance with these conjectures. © 2005 Elsevier B.V. All rights reserved. 238 1-4 290 293 Thurston, G., (1998) J. Clin. Invest., 101, p. 1401 Schmitt-Sody, M., (2003) Clin. Cancer Res., 9, p. 2335 Koeller, J.M., (1994) Ann. Pharmacother., 28, pp. S5 Cavalcanti, L.P., (2001), Ph.D Thesis, Universidade Estadual de Campinas - UNICAMP, BrazilDaillant, J., Gibaud, A., (1999) X-ray and Neutron Reflectivity: Principles and Applications, , Springer Berlin Heidelberg New York Als-Nielsen, J., (1994) Phys. Rep., 246, p. 251