Tesis
Evaluación del Efecto Ansiolítico del Extracto Hidroalcohólico de Flor de Granadilla (Passiflora ligularis) en Ratones (Mus musculus)
Fecha
2013-06-21Registro en:
Pallo Sarabia, Mayra Alejandra. (2013). Evaluación del Efecto Ansiolítico del Extracto Hidroalcohólico de Flor de Granadilla (Passiflora ligularis) en Ratones (Mus musculus). Escuela Superior Politécnica de Chimborazo. Riobamba.
Autor
Pallo Sarabia, Mayra Alejandra
Resumen
To test the anxiolytic effect of passion fruit flower (Passifloraligularis) in mice (Mus musculus), by means of the Exploratory Activity test (maze), Curiosity test, Motor Incapacitation test (Chimney) and Relaxing Activity test (Rota-Rod), which were performed 30 minutes after administration. The test was performed in the Biotherium of the School of Biochemistry and Pharmacy of ESPOCH. Eighteen mice were divided into six equal groups: D1, D2, D3 (net experimental groups), B (blank), E (chemical positive control), R (natural positive control). The groups: D1, D2, D3, received as treatment granadilla flower extract, at a concentration of 100%, 70%, 40% respectively. The natural positive control group received Passifloraedulis, the chemical positive control group received Diazepam, and the blank group received only vehicle. For the analysis of the data obtained, ANOVA (analysis of variance) and Tukey 99% tests were used. After applying the granadilla flower extract treatment, anxiolytic activity was evidenced in the three concentrations, with the most effective dose being the 100% concentration. It was also proved by the toxicological and histopathological study that the doses administered were not harmful and histologically safe on the pharmacokinetic organs involved (Stomach, Liver, Kidney). I thus conclude that the use of passion fruit flower extract as an anxiolytic agent is scientifically proven with the present research, and also that the secondary metabolites involved in this pharmacological capacity are phenolic compounds with flavonic prototype and alkaloids. I recommend that based on this scientific work, future research on Passifloraligularis be continued, which will contribute to improve the lifestyle of the patient with anxiety disorder.