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Chicken skeletal muscle-associated macroarray for gene discovery
(FUNPEC-EDITORA, 2010)
Macro- and microarrays are well-established technologies to determine gene functions through repeated measurements of transcript abundance. We constructed a chicken skeletal muscle-associated array based on a muscle-specific ...
Cytotoxic effects of crotamine are mediated through lysosomal membrane permeabilization
(PERGAMON-ELSEVIER SCIENCE LTD, 2008)
Crotamine, one of the main toxic components of Crotalus durissus terrificus venom, is a small non-enzymatic basic polypeptide, which causes hind limb paralysis and necrosis of muscle cells. it is well-known that several ...
Interethnic differences in ADMA concentrations and negative association with nitric oxide formation in preeclampsia
(ELSEVIER SCIENCE BV, 2010)
Background: Recent studies have suggested that impaired nitric oxide (NO) formation in preeclampsia may result from increased concentrations of an endogenous NO synthase inhibitor, the asymmetric dimethylarginine (ADMA). ...
Detection of Leishmania (Leishmania) infantum RNA in fleas and ticks collected from naturally infected dogs
(SPRINGER, 2011)
The occurrence of the insect vector (sand flies) with low rates of Leishmania infection, as well as autochthonous transmission in the absence of the natural vector in dogs, have been reported. These unexpected data suggest ...
Evaluation of the Frequency of Micronuclei in Exfoliated Cells from Oral Lesions Previously Identified by Toluidine Blue
(KARGER, 2011)
Objective: Patients using a removable prosthesis are susceptible to a variety of oral lesions that may progress to cancer. Toluidine blue (TB) staining is used to identify premalignant lesions, but the results are still ...
Effects of the Association between a Calcium Hydroxide Paste and 0.4% Chlorhexidine on the Development of the Osteogenic Phenotype In Vitro
(ELSEVIER SCIENCE INC, 2008)
The present study aimed to evaluate whether the association between a calcium hydroxide paste (Calen paste) and 0.4% chlorhexidine (CHX) affects the development of the osteogenic phenotype in vitro. With rat calvarial ...
A novel bradykinin potentiating peptide isolated from Bothrops jararacussu venom using catallytically inactive oligopeptidase EP24.15
(BLACKWELL PUBLISHING, 2008)
Characterization of the peptide content of venoms has a number of potential benefits for basic research, clinical diagnosis, development of new therapeutic agents, and production of antiserum. Here, we use a substrate-capture ...
Evaluation of Quantitative RT-PCR Using Nonamplified and Amplified RNA
(LIPPINCOTT WILLIAMS & WILKINS, 2010)
Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is a standard assay in molecular medicine for gene expression analysis. Samples from incisional/needle biopsies, laser-microdissected tumor cells and ...
Mycoplasma synoviae cell invasion: Elucidation of the Mycoplasma pathogenesis in chicken
(ELSEVIER SCI LTD, 2011)
Fluorochrome-labelled cells of two field isolates and Mycoplasma synoviae (Ms) were inoculated onto monolayer cultures of fluorochrome-labelled HEp-2 cells and monitored by confocal laser scanning microscopy (CLSM). Ms was ...
Clove and eugenol in noncytotoxic concentrations exert immunomodulatory/anti-inflammatory action on cytokine production by murine macrophages
(WILEY-BLACKWELLMALDEN, 2012)
Objectives The extract and essential oil of clove (Syzygium aromaticum) are widely used because of their medicinal properties. Eugenol is the most important component of clove, showing several biological properties. Herein ...