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Effect of surface hydrophobicity distribution on retention of ribonucleases in hydrophobic interaction chromatography
The effect of surface hydrophobicity distribution of proteins on retention in hydrophobic interaction chromatography (HIC) was investigated. Average surface hydrophobicity as well as hydrophobic contact area between protein ...
Enhanced bulk and superficial hydrophobicities of starch-based bionanocomposites by addition of clay
In this work, thermoplastic starch (TPS)-clay bionanocomposites were obtained by an innovative methodology using a combination of methodologies commonly used in the composites and nanocomposites preparations. The main ...
Prediction of retention time of cutinases tagged with hydrophobic peptides in hydrophobic interaction chromatography
(ELSEVIER SCIENCE BV, 2007-06)
Hydrophobic interaction chromatography (HIC) is an important technique for protein purification, which exploits the separation of proteins based on hydrophobic interactions between the stationary phase ligands and hydrophobic ...
Current insights on protein behaviour in hydrophobic interaction chromatography
This paper gives a summary of different aspects for predicting protein behaviour in hydrophobic interaction chromatography (HIC). First, a brief description of HIC, hydrophobic interactions, amino acid and protein ...
Yeast flotation viewed as the result of the interplay of supernatant composition and cell-wall hydrophobicity
(Elsevier B.V., 2003-06-15)
Flotation is a process of cell separation based on the affinity of cells to air bubbles. In the present work, flotability and hydrophobicity were determined using cells from different yeasts (Hansenulla polymorpha, ...
Predicting the behaviour of proteins in hydrophobic interaction chromatography: 2. Using a statistical description of their surface amino acid distribution
This paper focuses on the prediction of the dimensionless retention time (DRT) of proteins in hydrophobic interaction chromatography (HIC) by means of mathematical models based on the statistical description of the amino ...