Now showing items 1-10 of 4023
Versatile electroanalysis of cellular receptor: The case of Toll-like immune receptors evaluated on transfected human cell
In the present work we compare the results obtained by two approaches to detect receptor expression on cells surface: (i) the confocal laser scanning microscopy, which is an established methodology to study cell biology ...
Using the comet and micronucleus assays for genotoxicity studies: a review
Physical, chemical and biological agents can act in the DNA, resulting in mutation involved in cancer. Thus, genotoxic tests are required by regulatory agencies in order to evaluate potential risk of cancer. Among these ...
Study of the design and analytical properties of the lethality neutralization assay used to estimate antivenom potency against Bothrops asper snake venom
The lethality neutralization assay performed in mice is the standard recommended by the World Health Organization to estimate antivenom potency. The interpretation of its results without considering its analytical capacity ...
Development of pipette tip gap closure migration assay (s-ARU method) for studying semi-adherent cell lines
This work presents a pipette tip gap closure migration assay prototype tool (semi-adherent relative upsurge—s-ARU—method) to study cell migration or wound healing in semi-adherent cell lines, such as lymph node carcinoma ...
Biological control of Rhizoctonia solani in tomatoes with Trichoderma harzianum mutants
(Pontificia Universidad Católica de Valparaíso, 2010)
Conservación in vitro de chayote (Sechium edule)
In vitro conservation of chayote (Sechium edule). The in vitro medium-term conservation technique was assayed on chayote. Survival was assayed after the addition of high concentration of sucrose (0, 30, 50, 60, 70 y 80 ...
Cry1Ac and Vip3Aa proteins from Bacillus thuringiensis targeting Cry toxin resistance in Diatraea flavipennella and Elasmopalpus lignosellus from sugarcane
The biological potential of Vip and Cry proteins from Bacillus is well known and widely established. Thus, it is important to look for new genes showing different modes of action, selecting those with differentiated ...
(Humana Press, 2007-12)
Transcriptional feedback loops are at the core of the molecular clockworks. As single clock genes were cloned it was compelling to develop an assay that allowed simple and direct functional testing of putative activators ...